The network and system infrastructure are the basic framework of computerized system in the GLP laboratories, and their validation and management work is critical to the orderly operation of computerized systems in GLP organizations. The infrastructure can be validated during the system life cycle of a computerized system or independently. The validation of the infrastructure should take into account its specific aspects, such as infrastructure configuration, size and complexity, hardware and software, and network communications in order to achieve the validation of computerized system for regulatory purposes. Infrastructure validation methods, like system life cycle validation also involve the qualification or validation plans, requirementsspecification, qualification or validation reports, and operation and maintenance validation. This paper introduced in detail the feature of validation and management of infrastructure of computerized system so as to provide references for the establishment of computerized system that meet the requirements of GLP principles and regulations in China.