[关键词]
[摘要]
目的 研究氯霉素泼尼松搽剂抗Ⅳ型(迟发型)变态反应的药理作用。方法 C57BL/6/ICR健康小鼠随机分为对照组、基质组、模型组、莫米松乳膏组和氯霉素泼尼松搽剂高、中、低剂量组[分别给予高、中、低浓度(含氯霉素20、10、5 mg/mL,泼尼松1.00、0.50、0.25 mg/mL)的氯霉素泼尼松搽剂]。采用二硝基氯苯(DNCB)丙酮溶液诱发小鼠IV型变态反应,观察耳肿胀度、胸腺/脾脏指数;以4-氨基吡啶(4-AP)诱发小鼠皮肤局部瘙痒模型,观察舔体反应次数;用磷酸组织胺的累计剂量为指标测定小鼠致痒阈。结果 与模型组比较,氯霉素泼尼松搽剂各剂量组均能显著抑制小鼠耳肿胀度增加(P<0.05),显著抑制胸腺指数的增加(P<0.05),高剂量组能显著抑制脾脏指数的增加(P<0.05);中、高剂量组小鼠舔体反应次数显著下降(P<0.05);各剂量组的磷酸组织胺致痒阈显著高于模型组和基质组(P<0.05);且作用均呈剂量相关性。结论 氯霉素泼尼松搽剂局部外用具有良好的抗Ⅳ型变态反应和止痒作用,该制剂适用于临床具有Ⅳ型变态反应特征的皮损治疗。
[Key word]
[Abstract]
Objective To study the pharmacological effect of chloramphenicol prednisone liniment on hypersensitivity of type IV (delayed type). Method C57BL/6/ICR healthy mice were randomly divided into control group, matrix group, model group, mometasone cream group, chloramphenicol prednisone liniment low, medium and high dose groups [chloramphenicol prednisone liniment with high, medium and low concentrations (containing chloramphenicol 20, 10, 5 mg/ml, prednisone 1.00, 0.50, 0.25 mg/ml) was given respectively]. With allergy IV induced by DNCB (dinitrochlorobenzene) acetone solution, the effects of chloramphenicol prednisone liniment on allergy IV were observed by ear swelling, thymus index and spleen index. The local pruritus model of mice was induced by 4-aminopyridine (4-AP). The effects of licking times on the itching response were observed. The cumulative dose of histamine phosphate was used to determine the itching threshold of mice. Results Compared with the model group, chloramphenicol prednisone liniment can significantly inhibit the increase of ear swelling (P < 0.05), thymus index (P < 0.05), and spleen index (P < 0.05) in high dose group. The times of licking response decreased significantly in the middle and high dose groups (P < 0.05), the itching threshold of histamine phosphate in each dose group was significantly higher than that in the model group and matrix group (P < 0.05), and the effects were dose-dependent. Conclusion The topical application of chloramphenicol prednisone liniment has good anti-type IV allergic reaction and antipruritic effect. It is suitable for the treatment of skin lesions with type IV allergic reaction.
[中图分类号]
R965
[基金项目]
太和科研基金项目(2018JJXM057)