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[摘要]
目的 研究有氧运动对小鼠主动脉自噬相关因子表达及自噬活化的影响。方法 将8周龄C57BL/6雄性小鼠40只随机分为安静对照组(n=20)与有氧运动组(n=20),其中10只用于Real-Time PCR实验,10只用于Western-blot实验,有氧运动组小鼠进行6周的游泳运动(60 min/d,5 d/周),对照组小鼠自由活动。6周后取小鼠胸腹主动脉,采用Real-Time PCR方法检测自噬相关因子ULK1、Beclin1、LC3B mRNA水平;Western-blot方法检测主动脉LC3B蛋白中LC3B-II/LC3B-Ⅰ比值及p62蛋白水平。结果 与对照组相比,有氧运动组小鼠主动脉ULK1、Beclin1、LC3B mRNA水平明显升高(P<0.05);有氧运动组小鼠主动脉LC3B-II/LC3B-Ⅰ比值增大(P<0.05),p62蛋白降解增加(P<0.05)。结论 有氧运动能够使主动脉ULK1、Beclin1、LC3B自噬相关因子表达增强,进而增强主动脉自噬活性。
[Key word]
[Abstract]
Objective To investigate the effect of aerobic exercise on the levels of autophagy-related markers and Autophagy activation in mouse aorta. Methods Twenty C57BL/6 male mice aged 8 weeks were randomly divided into sedentary control group and aerobic exercise group. The aerobic exercise group were subjected to swimming for 6 weeks, 60 min/d, 5 days a week. The Thoracic and abdominal aorta of mice were isolated and detected after 6 weeks. Real-Time PCR was adopted to measure the mRNA levels of autophagy-related markers, including ULK1, Beclin1 and LC3B. Western-blot was applied to measure the expression level of autophagy-related protein including the ratio of LC3B-II/LC3B-Ⅰ and p62. Results Compared with the control group, in the aerobic exercise group, the mRNA levels of ULK1, Beclin1 and LC3B and the ratio of LC3B-II/LC3B-Ⅰ were significantly increased (P<0.05), the protein degradation of p62 was significantly increased (P<0.05). Conclusion Aerobic exercise can increase the expression of ULK1, Beclin1 and LC3B in the aorta, and then enhance the aortic autophagy activity.
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[基金项目]
山东省高等学校科技计划项目(J18KB117);滨州医学院附属医院青年科研基金(2013QNKYJJ02)