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[摘要]
目的 通过建立苯酚胶浆所致的慢性盆腔炎大鼠模型,对癃清片治疗妇科炎症的作用特点和机制进行探讨。方法 将雌性SD大鼠子宫内注射苯酚胶浆造成大鼠慢性盆腔炎模型,造模7 d后分组,随机分为6组:空白对照组、模型对照组及癃清片高、中、低剂量(9.355 2、4.677 6、2.338 8 g生药/kg)组和千金片组,给药14 d。测定各组大鼠子宫肿胀率及肿胀抑制率、对各组动物子宫、阴道组织进行HE病理学观察以评价炎症反应程度。通过细胞凋亡TUNEL染色观察子宫内膜细胞凋亡程度,并采用免疫组化染色检测肿瘤坏死因子α(TNF-α)、白介素4(IL-4)和细胞黏附因子(ICAM-1)的表达。结果 与空白对照组相比,模型对照组子宫肿胀明显;与模型对照组相比,癃清片各剂量组治疗后子宫肿胀率均有所降低,尤以癃清片中剂量组为佳。与空白对照组相比,模型对照组子宫可见明显扩张,内膜充血明显,水肿程度严重,而给药各组中充血水肿现象少见。在炎性浸润方面,模型对照组阴道可见上皮细胞增生,表层出现黏液层,以中性粒细胞为主的炎细胞浸润,给药各组中炎细胞浸润程度较模型组轻。与空白对照组相比,模型对照组子宫细胞凋亡程度明显增加,癃清片能够明显改善子宫内膜细胞的凋亡程度。与空白对照组相比,TNF-α、IL-4及ICAM-1(CD54)蛋白表达均在胞浆及胞核,呈棕黄色,正常对照组大鼠子宫腺体及炎细胞可见弱阳性表达,模型组大鼠子宫腺体及炎细胞可见强阳性表达,其余各组表达均较模型组有不同程度减弱。结论 癃清片通过下调模型大鼠子宫组织中TNF-α、IL-4的表达水平,从而抑制子宫组织中ICAM-1的表达,防止和缓解子宫组织的黏连,达到治疗慢性盆腔炎的作用。
[Key word]
[Abstract]
Objective By establishing a rat model of chronic pelvic inflammation induced by phenol mortar, the characteristics and mechanism of Longqing Tablets in treatment of gynecological inflammation were discussed. Methods Chronic pelvic inflammation model in rats induced by intrauterine injection of phenol mortar in female SD rats. After 7 days of moulding, the chronic pelvic inflammation rats were randomly divided into 6 groups:blank control group, model group, high, medium and low doses of Longqing Tablets (9.355 2, 4.677 6, 2.338 8 g crude drug/kg) groups and Qianjin Tablets group. And all of groups were given drug for 14 d. The rate of uterine edema and its inhibition in each group were determined. The uterus and vagina tissues of each group were observed by HE pathology to evaluate the degree of inflammatory reaction. The degree of apoptosis of endometrium cells was observed by TUNEL staining, and the expression of TNF-α, IL-4, and ICAM-1 was detected by immunohistochemical staining. Results Compared with blank control group, the uterine swelling in the model group was significant. Compared with model group, the uterine swelling rate in each dose group of Longqing Tablets were decreased after treatment, especially in the middle dose group of Longqing Tablets. Compared with blank control group, the uterus of the model control group was obviously dilated, endometrial congestion was obvious, and the degree of edema was serious, while the phenomenon of congestion and edema was rare in each group. In terms of inflammatory infiltration, epithelial cell proliferation, mucus layer and neutrophil-dominated inflammatory cell infiltration were observed in the vagina of the model control group. The degree of inflammatory cell infiltration in each group was lighter than that in the model group. Compared with the blank control group, model group in the uterus cell apoptosis was significantly increased, Longqing Tablets could obviously improve the degree of the apoptosis of endometrial cells. Compared with the blank control group, TNF-α, IL-4 and ICAM-1 (CD54) proteins were all expressed in cytoplasm and nucleus, which were brownyellow. Week positive expression was observed in uterine glands and inflammatory cells of rats in the normal control group, while strong positive expression was observed in the model group, the expression of the remaining groups was decreased to different degrees compared with that of the model group. Conclusions Longqing Tablets by cutting the uterus tissue in rat models of TNF-α and IL-4 levels, thus inhibiting the uterus tissue cells ICAM-1 expression, to prevent and alleviate adhesion of uterus tissue, reach the role of the treatment of chronic pelvic inflammatory disease.
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