[关键词]
[摘要]
目的 研究特定聚合度原花青素为主要成分的葡萄籽提取物在腹泻治疗中的新用途。方法 将葡萄籽进行粉碎、粗提、精制并分级沉淀后得到6个部位,以大鼠腹泻模型评价葡萄籽原花青素提取物各部位的止泻活性,并对有效部位的总原花青素含量、平均聚合度和主要单体组成等特性进行分析。结果 来源于葡萄籽的多聚原花青素部位F4对应激性腹泻模型有显著活性,而其他纯化部位和总提物并无此作用。结论 F4部位在主要原花青素单体组成和含量上与总原花青素及其他部位相比并无差异,但在平均聚合度上差异明显,表明F4部位的止泻活性与其特定的聚合度密切相关。
[Key word]
[Abstract]
Objective The procyanidins was researched as the main component of grape seed extract in the treatment of diarrhea. Methods Grape seeds were crushed, roughly extracted, refined and then six parts were obtained after fractionation. Subsequently, a diarrhea model was used to study the antidiarrheal effectiveness of all parts of the extract. In addition, the characteristics of the total proanthocyanidin contents of active fractions, the mean degrees of polymerization, and the monomer composition were analyzed. Results The polymeric procyanidins site F4 derived from grape seeds on stress diarrhea model had significant activity, while other parts of the purification and the total extract did not have such effect. Conclusion F4 is nothing special compared with the total proanthocyanidins and other parts on the monomer composition and the content of procyanidins, but in significantly difference on the mean degree of polymerization. The results suggest that F4 shows antidiarrheal activity, and its specific was closely related to the degree of polymerization.
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[基金项目]
天津科市技计划项目(12JCQNJC08700)