[关键词]
[摘要]
目的 探讨坤泰胶囊对不同治疗方法下卵巢早衰大鼠卵巢功能和生殖功能的影响。方法 将84只SD雌性大鼠随机分为卵巢功能组和生殖功能组两个大组,分别观察卵巢功能和生殖功能,每个大组分为空白组、模型组和坤泰胶囊高、中、低剂量组及结合雌激素片组,采用ig雷公藤多苷片(75 mg/kg,14 d)建立卵巢早衰模型,造模结束后,分别用坤泰胶囊高、中、低剂量(1.2、0.6、0.3 g/kg,36 d)及结合雌激素片(0.075 mg/kg,36 d)进行治疗。治疗结束后,卵巢功能组所有大鼠用酶联免疫吸附试验(ELISA)法检测大鼠血清性激素水平的变化,用实时荧光定量PCR(qRT-PCR)法检测大鼠卵巢组织相关因子Fas、FasL mRNA的表达水平的变化。将生殖功能组所有雌性大鼠与12只SD雄性大鼠进行合笼,观察记录孕鼠一般情况及子鼠的生长发育情况,观察各组雌性大鼠的生殖能力。结果 治疗组与模型组比较,随着治疗天数的增加,大鼠的卵巢功能和生殖功能均明显改善,血清卵泡生成激素(FSH)、黄体生成激素(LH)水平明显降低,雌二醇(E2)、抗穆勒管激素(AMH)水平明显升高,FasmRNA表达明显升高、FasLmRNA表达明显降低,差异均有统计学意义(P<0.05);孕鼠摄食量及体质量均增长较快,差异有统计学意义(P<0.05)。大鼠产仔总数多,受孕率、妊娠率均较高,无明显子代小鼠畸形率,差异有统计学意义(P<0.05)。结论 坤泰胶囊能够改善卵巢早衰大鼠的卵巢功能和生殖功能,可修复育龄期大鼠卵巢功能病理性损伤,有效调节卵巢早衰大鼠血清性激素水平,上调FasmRNA表达,下调FasLmRNA表达,提高卵巢早衰大鼠受孕率及妊娠率,对子代小鼠无明显的致畸性。
[Key word]
[Abstract]
Objective To investigate the effects of Kuntai Capsule on ovarian function and reproductive function in rats with premature ovarian failure under different treatments. Methods 84 SD female rats were randomly divided into two groups:ovarian function group and reproductive function group. The ovarian function and reproductive function were observed. Each group was divided into blank group, model group and Kuntai capsule high, medium, low dose group and conjugated estrogen tablet group. The premature ovarian failure model was established by Ig Tripterygium glycoside tablet (75 mg/kg, 14 days). After the model was established, Kuntai capsule high dose group was used. Medium and low doses (1.2, 0.6, 0.3 g/kg, 36 days) and conjugated estrogen tablets (0.075 mg/kg, 36 days) were administered. After treatment, all rats in ovarian function group were tested for the changes of serum sex hormone levels by ELISA, and the expression levels of Fas and FasL in ovarian tissue were detected by real-time fluorescence quantitative PCR (qRT-PCR). All female rats in reproductive function group were caged with 12 SD male rats. The general situation of pregnant rats and the growth and development of offspring were observed and recorded, and the reproductive ability of female rats in each group was observed. Results Compared with the model group, the ovarian function and reproductive function of rats were improved significantly with the increase of treatment days. The levels of serum follicle-forming hormone (FSH) and luteinizing hormone (LH) were significantly decreased, the levels of estradiol (E2) and anti-Mullerian hormone (AMH) were significantly increased, the expression of Fas gene was significantly increased, and the expression of FasL gene was significantly decreased in the treatment group (P The intake and body mass of pregnant rats increased rapidly, and the difference was statistically significant (P<0.05). The total number of offspring of rats was large, the pregnancy rate and pregnancy rate were high, and there was no obvious deformity rate in offspring of mice, the difference was statistically significant (P<0.05). Conclusion Kuntai Capsule can improve the ovarian function and reproductive function of premature ovarian failure rats, repair the pathological damage of ovarian function in reproductive age rats, effectively regulate the level of serum sex hormones, up-regulate the expression of Fas gene, down-regulate the expression of FasL gene, improve the pregnancy rate and pregnancy rate of premature ovarian failure rats, and has no obvious teratogenicity to offspring mice.
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[基金项目]
国家中医药管理局全国名老中医药专家高慧传承工作室建设项目(编号2015048157);河北省省级重大医学科研项目(编号zyzd2013018);河北省政府资助临床医学优秀人才培养项目(编号2014571024);河北省中医药管理局中医药类科研计划课题(编号2016068)