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[摘要]
目的 建立高效液相色谱法测定田七痛经胶囊中阿魏酸、藁本内酯、欧当归内酯A的方法。方法 选用Thermo Acclaim C18色谱柱,Thermo SCIENTIFICSyncronis C18色谱柱;检测波长为321、280 nm;体积流量1.0 mL/min;柱温35℃结果 阿魏酸、藁本内酯、欧当归内酯A的质量浓度与峰面积分别在1.12~112.30、1.33~132.60、0.96~95.50 μg/mL呈良好的线性关系;回归方程分别为Y=46 335 540 X+44 186.71(r=0.998),Y=14 514 060 X+8 715.27(r=0.999),Y= 22 031 250 X+16 472.10(r=0.999)。结论 该方法准确,简便,灵敏,可作为田七痛经胶囊质量控制的方法。
[Key word]
[Abstract]
Objective Establishing aRP-HPLC method for the determination of the contents of Ferulic acid, Ligustilide and Levistolide A in tianqi tongjing capsule. Methods Thermo Acclaim C18 column,Thermo SCIENTIFIC Syncronis C18 column was used. The detection wavelength was set at 321 nm, 280 nm. The flow rate was 1.0 mL/min. The column temperature was maintained at 35℃. Results Ferulic acid was linear in the range from 1.12 to 112.30 μg/mL and the regression equation was Y=46 335 540 X + 44 186.71 (r=0.998). Ligustilide was linear in the range from 1.33 to 132.60 μg/mL and the regression equation was Y=14 514 060 X + 8 715.27 (r=0.999). Levistolide A was linear in the range from 0.96 to 95.50 μg/mL and the regression equation was Y=22 031 250 X + 16 472.10 (r=0.999). Conclusion The method is accurate, simple and feasible, and can be used as an ideal method for the quality control of tianqi tongjing capsule.
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国家评价性抽验项目