目的 研究小分子阿胶对小鼠免疫功能的调节作用。方法 将ICR小鼠随机分为对照组、模型组、生血丸3.00 g/kg组和小分子阿胶3.00、1.50、0.75 g/kg组，ig给药14 d，ip环磷酰胺或氢化可的松建立免疫低下模型，用鸡红细胞诱导小鼠溶血素，测定小鼠血清溶血素水平，观察小分子阿胶对体液免疫的影响；采用二硝基氯苯诱导小鼠耳廓肿胀，检测小鼠耳廓肿胀度，观察小分子阿胶对细胞免疫的影响；利用流式细胞术对小鼠淋巴细胞亚群进行分析，观察小分子阿胶对小鼠淋巴细胞分型的影响。结果 与模型组比较，小分子阿胶3.00、1.50、0.75 g/kg显著提高免疫功能低下小鼠血清溶血素含量（P<0.01）；3.00、1.50 g/kg显著提高小鼠耳廓肿胀度（P<0.01、0.05）；3.00 g/kg显著提高小鼠T淋巴细胞（CD3⁺）、辅助性T细胞和迟发超敏性T细胞（CD3⁺CD4⁺）占淋巴细胞的比例（P<0.01）。结论 小分子阿胶能提高小鼠体液免疫和细胞免疫功能，对免疫功能有正向调节作用。

Objective To study the effect of small molecular Asini Corii Colla (SMACC) on immune function in mice. Methods ICR mice were randomly divided into control group, model group, Shengxue Pills (3.00 g/kg) group, and SMACC (3.00, 1.50, 0.75 g/kg) group, and they were ig administered for 14 d. Immunosuppressive model was established by intraperitoneal injection of cyclophosphamide or hydrocortisone. The use of red blood cells in chicken was to induce the production of hemolysin in mice. The content of serum hemolysin was measured to determine the effect of SMACC on humoral immunity. The ear swelling of mice was induced by two nitrochlorobenzene, and the auricular swelling of mice was detected to observe the effect of SMACC on cell immunity. Lymphocyte subsets in mice were analyzed by flow cytometry to observe the effect of SMACC on lymphocyte subtyping. Results Compared with model group, SMACC significantly increased the serum hemolysin content at doses of 3.00, 1.50, and 0.75 g/kg (P < 0.01), significantly increased the degree of auricular swelling in mice at doses of 3.00 and 1.50 g/kg (P < 0.01 and 0.05), and significantly promoted the proportion of T lymphocytes (CD3⁺), helper T cells, and delayed hypersensitivity T cells (CD3⁺CD4⁺) at doses of 3.00 g/kg (P < 0.01). Conclusions SMACC can enhance humoral immunity and cellular immune function in mice and has a positive regulatory effect on immune function in mice.

国家科技重大专项（2015ZX09501004）；天津市科技计划项目（16PTGCCX00090）；国家中药标准化项目—阿胶标准化建设（ZYBZH-Y-SD-31）；泰山产业领军人才工程专项经费资助