[关键词]
[摘要]
目的 建立小鼠腹水瘤模型,研究其临床指标的变化情况,探讨小鼠癌性腹水模型的特征。方法 制备小鼠肝癌(H22)、骨肉瘤(S180)腹水瘤模型,观察腹水生长及小鼠的体质量、进食、饮水量、自主活动等一般状态;待腹水生长至第12天,眼眶采血、抽取腹水,应用全自动血液分析仪、生化分析仪、电解质分析仪进行血液学、血清生化、腹水常规、生化及电解质检查;取少量腹水涂片后瑞氏染色镜检;使用试剂盒测定血清及腹水中乳酸(LD)水平。结果 小鼠腹水形成率为100%,腹部逐渐膨胀明显,活动变得迟缓,毛发不荣,进食及饮水量均减少;与对照组比较,H22和S180组小鼠于腹水接种第4天起体质量显著升高(P<0.01);血中白细胞(WBC)数量显著升高(P<0.01),红细胞(RBC)、血小板(HGB)呈不同程度的降低;腹水中少见血象细胞;丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、尿素氮(BUN)、乳酸脱氢酶(LDH)、LD水平显著升高,肌酐(CREA)、白蛋白(ALB)、血糖(GLU)水平显著降低(P<0.05、0.01);电解质紊乱。结论 H22、S180小鼠腹水瘤模型多种检测指标异常,且与临床表现基本一致,具有典型的恶性腹水特征。
[Key word]
[Abstract]
Objective To study the changes of clinical indicators in mice with ascites tumor, and to explore the characteristics of the model of cancerous ascites in mice. Methods H22 and S180 ascites tumor model of mice was established. The general state of ascites growth, body mass, feeding, drinking water and autonomous activity were observed. After the ascites grew to 12 days, hematology, serum biochemistry, ascites cells, biochemistry and electrolytes were examined by automatic hematology analyzer, biochemical analyzer and electrolyte analyzer. A small amount of ascites smear was taken and reelson staining microscopy was used. The LD levels in serum and ascites were measured with kits. Results The incidence of ascites in mice was 100%, the abdominal swelling was obvious, the activity became slow, the hair was not proud, and the amount of food and drinking water decreased. Compared with control group, the body mass of H22 and S180 mice increased significantly (P<0.01) after fourth days of ascites. The number of WBC in the blood of H22 and S180 mice increased significantly (P<0.01), and the RBC and HGB were reduced in varying degrees. The hemogram cells were rare in the ascites. The levels of ALT, AST, BUN, LDH and LD in the blood and ascites all increased, and the levels of CREA, ALB and GLU were all decreased (P<0.05 and 0.01) and electrolyte disturbance. Conclusion H22 and S180 mouse ascites tumor model has a variety of abnormal detection indexes, which are consistent with the clinical manifestations, and have typical malignant ascites characteristics.
[中图分类号]
[基金项目]