目的 观察骨碎补总黄酮对大鼠诱导膜中血管内皮生长因子（VEGF）、骨形态发生蛋白-2（BMP-2）表达的影响。方法 60只3月龄SD大鼠，随机分为3组：模型组、填充组和填充+TFDR组，每组20只，建立大鼠长段骨缺损模型，模型组骨缺损处仅克氏针固定，填充组及填充+TFDR组骨缺损处用抗生素骨水泥填充，并用克氏针固定；填充+TFDR组ig给予TFDR （67.5 mg/kg），模型组及填充组ig等量生理盐水，每天给药1次，连续6周。给药结束后，处死大鼠，切取骨水泥周围包绕的白色膜状物，模型组切取对应阶段的瘢痕组织，Elisa定量及免疫组化法观察诱导膜中BMP-2、VEGF表达变化。结果 填充组BMP-2、VEGF表达水平显著高于模型组，填充+TFDR组BMP-2、VEGF表达水平均显著高于填充组和模型组，各组间差异均有统计学意义（P<0.05）；免疫组化检测结果显示相同趋势。结论 TFDR促进Masquelet技术诱导膜中BMP-2和VEGF表达。

Objective To observe the effects of total flavonoids of Drynariae Rhizoma (TFDR) on the expression of vascular endothelial growth factor (VEGF) and bone morphogenetic protein (BMP-2) in rat Masquelet-induced membrane.Methods Sixty SD rats aged 3 months were randomly divided into three groups:model group, filling group, and filling + TFDR group with 20 rats in each group. Rat long segment bone defect model was established. The bone defects in model group were fixed only by Kirschner wire, and were filled with antibiotic bone cement in filling group and filling + TFDR group. Rats in filling + TFDR group were ig given TFDR (67.5 mg/kg), the model group and the filling group were treated with equivalent saline, once a day for six weeks. The rats were sacrificed after administration, and bone cement and scar tissue in model group were removed. The expression of BMP-2 and VEGF in the induced membrane was observed by immuno-histochemical analysis of the filling tissue and the induced membrane.Results The positive expression of BMP-2 and VEGF in filling group were higher than model group by Elisa quantitative detection, which of the filling + TFDR group were significantly higher than model group and filling group, the difference was statistically significant by statistical analysis (P < 0.05). Immuno-histochemical analysis showed the same trend.Conclusion TFDR can promote the expression of BMP-2 and VEGF in Masquelet-induced membrane.

河南省中医药科学研究专项课题资助项目（2015ZY02062）