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[摘要]
目的 建立芪柏颗粒的UPLC特征图谱,为评价不同批次成品的质量提供参考。方法 采用ACQUITY UPLC HSS T3 C18(100 mm×2.1 mm,1.8 μm)作为色谱柱,以乙腈-0.3%甲酸水为流动相,梯度洗脱,柱温28℃,波长254 nm,体积流量0.4 mL/min。结果 建立芪柏颗粒的UPLC特征图谱,12批不同批次成品相似度均>0.92,共标定46个共有峰,筛选其中25个共有峰与单味药材进行比对并归属,其中4个共有峰来源牡丹皮,4个共有峰来源甘草,2个共有峰来源炙黄芪,2个共有峰来源墨旱莲,1个共有峰来源卷柏,12个共有峰未进行归属。结论 成功建立芪柏颗粒的UPLC特征图谱分析方法,其精密度、重复性和稳定性良好,可为芪柏颗粒质量标准提升作参考。
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[Abstract]
Objective To establish a UPLC characteristic chromatogram of Qibai Granule for quality control. Methods The ACQUITY UPLC HSS T3 C18 column (100 mm×2.1 mm, 1.8 μm) was used with a mobile phase comprised of acetonitrile-0.3% formic acid for gradient elution. The column temperature was 28℃. The detection wavelength was 254 nm. The flow rate was 0.4 mL/min. Results The UPLC characteristic chromatogram of Qibai Granule was established. The similarities of 12 batches of Qibai Granule were above 0.92. A total of 46 common peaks were found, where 25 common peaks were filtered for belonging to seven medicinal herbs. Among them, four mutual peaks were from Moutan cortex, four mutual peaks were from Glycyrrhizae radix et rhizoma, two common peaks came from Astragali radix praeparata cum melle, two common peaks came from Ecliptae herba, one common peak came from Selaginellae herba, and twelve were unknown sources. Conclusion The method is accurate, repeatable and stable, which can provide the reference for the study of quality control.
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