[关键词]
[摘要]
目的 探讨复方HD对环磷酰胺诱导的免疫抑制小鼠的免疫增强作用。方法 ICR小鼠分别以蒸馏水(对照组、模型组)、香菇多糖(200 mg/kg)、复方HD(5、10、20 g/kg)每天1次ig给药,连续10 d;除对照组外,在第4~6天ip环磷酰胺(40 mg/kg)制备免疫抑制小鼠模型(对照组除外);每天给药前精确称量并记录小鼠体质量。于最后一次给药24 h后,测定小鼠脾指数、胸腺指数;流式细胞术进行全血中T细胞亚群分析;ELISA法检测血浆中IFN-γ和IL-4水平。结果 与模型组比较,复方HD低、中、高剂量组显著提高小鼠的胸腺指数,CD3+、CD3+CD4+细胞数以及CD4+/CD8+比值(P<0.05),显著恢复血浆中IFN-γ水平和IFN-γ/IL-4比值(P<0.05),纠正了Th1/Th2偏移,并呈现出一定的浓度相关性。结论 复方HD能够促进环磷酰胺诱导的免疫抑制小鼠免疫功能的恢复,证明其在预防及治疗免疫功能低下方面具有一定的疗效。
[Key word]
[Abstract]
Objective To systematically elucidate the in vivo immune-enhancement effects of Fufang HD in immunosuppressed mice induced by cyclophosphamide treatment. Methods ICR mice received gastric administration of physiological saline (control group), lentinan (200 mg/kg), Fufang HD low-, medium-, and high-dose (5, 10, and 20 g/kg) once daily for 10 consecutive days, respectively, accompanied by intraperitoneal injection of cyclophosphamide (40 mg/kg) on days 4-6. Accurately weighing and recording the body mass of mice every day. After 24 h of the last administration, spleen index and thymus index of mice were detected. Flow cytometry was used to analyze T cell subsets in whole blood, and the levels of IFN-and IL-4 in plasma were detected by ELISA. Results Compared with model group, low-, medium-, and high-dose Fufang HD treatment accelerated recovery dose-dependently of thymus index, lymphocytic percentages of CD3+, CD4+, and CD4+/CD8+ as well as restored the level of serum INF-γ (P<0.05) and corrected Th1/Th2 deviation. Conclusions The results demonstrate that treatment with Fufang HD results in accelerating recovery of immunosuppression in Cy-treated mice for the first time, which is competent in taking into consideration for both precautions and remedy.
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[基金项目]
辽宁省科学技术计划项目(2014226033);中国医科大学校内课题基金项目(XZR20160007);中国医科大学附属第一医院院内基金项目(FSFH201717);中国医科大学附属第一医院药学部科室基金