目的 研究艾叶制炭前后对人主动脉内皮细胞组织因子途径抑制物（TFPI）、内皮细胞血管性假血友病因子（vWF）、NO含量的影响，以及对大鼠血小板氨基末端激酶-1（JNK1）表达水平的影响，阐明艾叶炭止血机制。方法 将体外培养的人主动脉内皮细胞（HEAC）随机分组，分别给予不同极性（石油醚、二氯甲烷、醋酸乙酯、正丁醇、水、水提物、鞣质、总黄酮）部位的艾叶生、炭品，终质量浓度为200、100、50 μg/mL，培养48 h后，取细胞培养液试剂盒法测定TFPI、vWF、NO含量；采用免疫蛋白印迹方法检测艾叶制炭前后各部位对大鼠血小板JNK1磷酸化水平的影响。结果 与对照组及相应生品组比较，艾叶炭品二氯甲烷和鞣质部位TFPI、NO含量显著降低，vWF含量显著升高（P<0.01）；大鼠血小板JNK1磷酸化水平显著升高（P<0.01）。与对照组比较，艾叶鞣质部位TFPI、NO含量显著降低，vWF含量显著升高（P<0.01）；大鼠血小板JNK1磷酸化水平显著升高（P<0.01）。结论 二氯甲烷和鞣质部位是艾叶炭的主要止血作用部位，其止血机制为降低TFPI、NO含量、增加vWF的含量；明显提高血小板JNK1的磷酸化水平。

Objective To investigate the effect of Artemisiae argyi Folium being charred on the content of TFPI, vWF, NO in HAEC, and JNK1, and to clarify the haemostatic mechanism of Artemisia argyicarbonisatus and part of the mechanism of platelet activation. Method The HEAC cultured in vitro were randomly divided into groups, which were given different polar parts of Artemisiae argyi Folium and Artemisia argyicarbonisatus with final concentration of 200, 100, and 50 μg/mL. The content of of TFPI, vWF, NO in cell culture fluid were measured by Kit after HEAC were cultured 12 h, measured the effect of Artemisiae argyi Folium before and after being charred on rat platelet JNK1 phosphorytion level. Results Compared with control group and the corresponding raw products group, the content of TFPI and NO in dichloromethane and tannin of Artemisia argyicarbonisatus decreased significantly, the content of vWF increased significantly (P<0.01), and the level of platelet JNK1 phosphorylation in rats increased significantly (P<0.01). Compared with the control group, the content of TFPI and NO in tannin of Artemisiae argyi Folium decreased significantly, and the content of vWF increased significantly (P<0.01), and the level of platelet JNK1 phosphorylation in rats increased significantly (P<0.01). Conclusion Dichloromethane and tannin sites are the main hemostasis part of Artemisia argyicarbonisatus, its hemostatic mechanism is to reduce the content of TFPI and NO, increase the content of vWF, and increase the level of phosphorylation of platelet JNK1 obviously.

艾叶炭炮制原理研究国家自然基金资助项目（81173545）