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[摘要]
目的 研究阿胶对免疫低下小鼠免疫功能的影响。方法 小鼠ip环磷酰胺或氢化可的松建立免疫低下模型,高、中、低(3.00、1.50、0.75 g/kg)剂量阿胶ig给予小鼠14 d后,测定血清溶血素水平,观察阿胶对体液免疫的影响;进行二硝基氯苯诱导的小鼠耳廓肿胀实验,检测小鼠耳廓肿胀度,观察阿胶对细胞免疫的影响;流式细胞术检测阿胶对淋巴细胞亚群的影响;进行碳廓清实验,检测廓清指数K和吞噬指数α,观察阿胶对非特异性免疫的影响。结果 与模型组比较,阿胶3.0、1.5、0.75 g/kg均可显著提高免疫功能低下小鼠血清溶血素含量(P<0.001);3.0、1.5 g/kg能明显提高小鼠耳廓肿胀度(P<0.01、0.001);3 g/kg能显著提高小鼠CD3+(T淋巴细胞)、CD3+CD4+(辅助性和迟发超敏性T细胞)占淋巴细胞百分比(P<0.05);阿胶对小鼠的碳粒廓清指数K和吞噬指数α无显著影响。结论 阿胶能显著提高免疫低下小鼠的体液免疫和细胞免疫,提高CD3+、CD3+CD4+阳性细胞比例,对非特异性免疫无明显影响。
[Key word]
[Abstract]
Objective To study the effect of Asini Corii Colla (ACC) on immune function in hypoimmune mice. Methods ACC of 3.00, 1.50 and 0.75 g/kg was given to hypoimmune model mice induced by Cyclophosphamide or Hydrocortisone for 14 d. The serum hemolysin content were measured to determine the effect of ACC on humoral immunity. The mouse auricle swelling test induced by two nitrochlorobenzene was carried out to detect the swelling of the auricle in mice, and the effect of ACC on the cell immunity was observed. Flow cytometry was used to detect the effect of ACC on lymphocyte subsets. The carbon clearance experiment was carried out to detect the clearance index K and phagocytic index α, and the effect of ACC on non-specific immunity was observed. Results Compared with model group, ACC could significantly promote the serum hemolysin content (P<0.001) at dose of 3.00, 1.50, and 0.75 g/kg, significantly increase the swelling of auricle in mice (P<0.01, 0.001) at doses of 3.00 and 1.50 g/kg, and significantly increase percentage of CD3+ (T lymphocytes), CD3+CD4+ (helper T cells and delayed hypersensitive T cells) at dose of 3.00 g/kg. There was no significant effect on the phagocytic index K and the clearing index α. Conclusion ACC can stimulate specific immune function and promote the proportion of CD3+, CD3+CD4+ positive cells, it has no obvious effect on non-specific immunity.
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[基金项目]
国家科技重大专项(2015ZX09501004);天津市科技计划项目(16PTGCCX00090);山东省重点研发计划项目(2016GGH4514);国家中药标准化项目(ZYBZH-Y-SD-31)