目的 对阿胶的补血、抗疲劳、抗氧化、止血的功效进行研究。方法 将SD大鼠随机分为对照组、模型组、生血丸1.5 g/kg组和阿胶1.500、0.750、0.375 g/kg组，ig给药14 d，采用乙酰苯肼联合环磷酰胺制备大鼠复合血虚模型，检测大鼠游泳力竭时间，进行血液学及血清丙二醛（MDA）、超氧化物歧化酶（SOD）水平检测，考察阿胶的补血、抗疲劳、抗氧化作用；ICR小鼠随机分为对照组、生血丸3 g/kg组和阿胶3.00、1.50、0.75 g/kg组，ig给药15 d，以毛细管法和剪尾法测定凝血时间（CT）和出血时间（BT）；采用肝素化大鼠出血模型考察阿胶的止血作用及可能机制。结果 在复合血虚模型中，与模型组比较，阿胶1.50、0.75 g/kg能够明显延长大鼠游泳时间（P<0.05、0.01），体力耗竭速度减慢；1.5 g/kg可升高红细胞计数（RBC）、血红蛋白（HGB）浓度、红细胞压积（HCT）、淋巴细胞（LYM）百分比（P<0.05）；1.500、0.750、0.375 g/kg显著降低血清中MDA的含量（P<0.01）。在正常血液功能检测中，3.00、1.50 g/kg阿胶能够显著缩短CT和BT（P<0.05），改善率最大可达29.4%。在肝素化出血模型中，1.5 g/kg阿胶可显著逆转肝素化所致的凝血酶原时间（PT）延长（P<0.05）。结论 阿胶具有"升红"、提高免疫力、抗氧化、抗疲劳的药理活性，且能够拮抗血液的肝素化，对凝血因子可能具有活化作用，起到止血收敛的作用。

Objective To evaluate the antifatigue, anti-oxidant and hemostatic effects, and to explore the potential hemostatic mechanism of Asini Corii Colla (ACC). Methods The SD rats were randomly divided into control group, model group, Shengxue Pills (1.5 g/kg) group and ACC (1.500, 0.750, and 0.375 g/kg) group, and rats were ig administered for 14 d. Rat model of complex blood deficiency induced by acetyl phenylhydrazine (APH) and cyclophosphamide (CTX) was used to detect rat exhaustive swimming time, take hematological examination, Malondialdehyde (MDA), and superoxide dismutase (SOD) level detection, which aims to explore the hematopoiesis, antifatigue and anti-oxidant effects of ACC. ICR mice were randomly divided into control group, Shengxue Pills (3 g/kg) group and ACC (3, 1.50, and 0.75 g/kg) group, and they were ig administered for 15 d. Clotting time (CT) and the bleeding time (BT) in normal mice were measured using methods of tail cutting and glass capillary. The heparinized bleeding model in rats was established for investigating the possible mechanism of hemostasis. Results In the complex model of blood deficiency, the ACC of 1.50 and 0.75 g/kg dose significantly prolonged the swimming time of model rats (P<0.05 and 0.01), delayed physical exhaustion time, increased the RBC, Lymphocyte, and HGB significantly (P<0.05), and decreased the content of MDA in serum significantly (P<0.05). In the normal blood function tests, the ACC significantly reduced the BT and CT (P<0.05), and the maximum improving rate was up to 29.4%. In hemorrhage model of heparinized blood, the ACC markedly reversed the prolonged prothrombin time (PT) induced by heparin (P<0.05). Conclusions The ACC have the pharmacological effects of hematopoiesis, immunity enhancement, anti-oxidation, and antifatigue. Additionally, the ACC could also antagonize the blood heparinization by the activation of coagulation factors in a certain extent, so ACC may play a role in hemostasis.

国家科技重大专项（2015ZX09501004）；天津市科技计划项目（16PTGCCX00090）；山东省重点研发计划（2016GGH4514）；国家中药标准化项目（ZYBZH-Y-SD-31）