目的 探讨刺五加多糖（ASPs）对刀豆蛋白A（Con A）造成的免疫性肝损伤模型的保护作用。方法 将BALB/c小鼠随机分成7组，对照组，模型组，ASPs低、中、高剂量（36.25、72.50、145.00 mg/kg）组，联苯双酯滴丸（阳性药，200 mg/kg）组，吡咯烷二硫代氨基甲酸盐（PDTC，NF-κB抑制剂/抗氧化剂，200 mg/kg）组。ASPs、联苯双酯滴丸组每天ig给药1次，连续7 d；对照组、模型组和PDTC组以0.1 mL/10 g生理盐水ig给药。于末次ig后，PDTC组ip PDTC，再于0.5 h后，除对照组外均尾iv 20 mg/kg Con A诱导肝损伤。8 h后处死动物取材，试剂盒法检测血清中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT），血清、脾脏组织中白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、干扰素-γ（IFN-γ）的蛋白含量；实时荧光定量PCR（qRT-PCR）法检测脾脏组织中IL-2、IL-4、IFN-γ mRNA表达。结果 与模型组比较，ASPs显著降低血清中AST、ALT，IL-2、IL-4、INF-γ血清、脾脏组织中的蛋白含量及脾脏组织中mRNA的表达（P<0.05、0.01），且均呈剂量相关性。结论 ASPs降低转氨酶活性、减少炎性细胞因子的分泌和表达，对免疫性肝损伤有一定的保护作用。

Objective To discuss the protective effect of Acanthopanax senticosus polysaccharides (ASPs) by using of Con A causing immunological liver injury model. Method Seventy mice were randomly divided into seven groups, named as control group, model group (Con A), low-, medium-, and high- dose (36.25, 72.5, and 145 mg/kg) groups of ASPs, Bifendate (20 mg/kg, positive drug) group and PDTC (NF-kappa B inhibitor/anti-oxidant, 200 mg/kg) group. The treatment group and positive control group were ig given ASPs and bifendate once daily for 7 d. Control group, model group and PDTC group were ig given normal saline once daily for 7 d. And at the last time, the PDTC group were ip injected PDTC (200 mg/kg). After 0.5 h, immunological liver injury was induced by Con A (20 mg/kg) by injecting via the tail vein in mice. Control group injected the same dose of normal saline. The mice were killed in 8 h later as well as taken tissues and blood using for detecting indexes. Serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were detected by kits. The contents of interleukin-2 (IL-2), interleukin-4 (IL-4), and interferon gamma (IFN-γ) in serum and spleen tissues were detected. Real time fluorescence quantitative PCR (qRT-PCR) was used to detect IL-2, IL-4, and IFN-γ expression in spleen tissues. Results Compared with model group, ASPs not only could reduce the activity of ALT and AST, but also decrease the content of IL-2, IL-4, INF-γ, and RNA expression siginificanly (P<0.05 and 0.01). Conclusion Results showed that ASPs not only can decrease transaminases activity, but also reduce the secretion and RNA expression of inflammatory cytokines. Consequenly, ASPs has a protective effect on immunological liver injury.

黑龙江省博士后资助项目（LBH-Z16196）；黑龙江省应用技术研究与开发计划项目（2013G1031）