目的 动态观察组分配伍四逆汤对甲减大鼠下丘脑-垂体-甲状腺轴的影响。方法 ig 0.1%丙基硫氧嘧啶（PTU）混悬液法分别造模6、11、16、20周，用左甲状腺素钠片（9 μg/kg）及组分配伍四逆汤（生药9.6 g/kg）对模型动物治疗4周，酶联免疫吸附法测定大鼠血清三碘甲状腺原氨酸（T₃）、甲状腺素（T₄）、促甲状腺激素（TSH）、促甲状腺素释放激素（TRH）水平的变化。结果 甲减程度随着造模时间的延长而加重。造模6、11、16周甲减大鼠与对照组比较，模型组大鼠血清T₃、T₄、TRH水平显著降低（P<0.05、0.01），TSH水平显著升高（P<0.05、0.01）；与模型组比较，左甲状腺素钠片组及组分配伍四逆汤组大鼠血清中T₃、T₄、TRH水平均显著升高（P<0.05、0.01），TSH水平显著降低（P<0.05、0.01）。造模20周甲减大鼠与对照组比较，模型组大鼠血清T₃、T₄、TRH水平显著降低（P<0.01），TSH水平明显升高（P<0.01），左甲状腺素钠片组和组分配伍四逆汤组血清T₃、T₄水平均显著降低（P<0.05、0.01）；与模型组比较，左甲状腺素钠片组及组分配伍四逆汤组大鼠血清T₃、T₄无统计学差异，TRH水平显著升高（P<0.05、0.01），TSH水平显著降低（P<0.01）。结论 下丘脑-垂体-甲状腺轴在甲减发展过程中整体功能在退化，组分配伍四逆汤的调节作用是动态变化的，应在初期及早给予。

Objective To investigate the dynamic changes of component compatibility Sini Decoction on hypothalamic-pituitary-thyroid axis in hypothyroidism rats. Methods The hypothyroidism model of 6, 11, 16, and 20 weeks were established by 0.1% propylthiouracil (PTU) solution. Rat were randomly divided into model group, Sini Decoction group (9.6 g/kg; 4 weeks), and positive control group (Euthyrox 9 μg/kg; 4 weeks). ELISA method was used to determine the serum T₃, T₄, TSH, and TRH levels. Results The degree of hypothyroidism increased with the extension of time. Compared with control group, serum T₃, T₄,and TRH levels in model group of 6, 11 and 16 weeks hypothyroidism rats were significantly decreased (P<0.05 or 0.01), while the TSH level was increased (P<0.05 or 0.01). Compared with model group, serum T₃, T₄, and TRH contents were notably increased (P<0.05 or 0.01), and serum TSH content was decreased (P<0.05 or 0.01) in Sini Decoction group and positive control group. Compared with control group, serum T₃, T₄, and TRH levels in model group of 20 weeks were significantly decreased (P<0.01) and the TSH level was increased (P<0.01). However, the serum T₃ and T₄ contents of Sini Decoction group and positive control group were markedly decreased (P<0.05 or 0.01). Compared with model group, there were no statistically significant differences in the serum T₃ and T₄ contents in Sini Decoction group and positive control group, while the TRH contents were notably increased (P<0.05 or 0.01) and TSH level was decreased (P<0.01). Conclusion Total function of hypothalamic-pituitary-thyroid axis degenerated in the process of hypothyroidism, and the regulating effect of component compatibility Sini Decoction dynamically changed, which should be given as soon as possible in the early stage.

国家自然科学基金自助项目（81373546）