[关键词]
[摘要]
目的 研究金莲花总黄酮的吸收特征,为确定金莲花的主要药效物质和进一步开发利用提供依据。方法 建立外翻肠囊模型,浴槽中给予Tyrode液配置的质量浓度分别为2、6、10 mg/mL的金莲花总黄酮溶液,分别在给药后15、30、45、60、90、120、150、180 min从肠囊内取样100 μL,补以相同体积的空白Tyrode液,样品经处理后作为供试品溶液;以荭草素为对照品,应用酶标仪测定供试品在341 nm下的吸光度(A)值,计算总黄酮含量、单位面积累积吸收量(QS)和药物吸收率(V)。结果 浴槽中给予低、中、高浓度的总黄酮溶液后,随着时间的延长,肠囊内V逐渐增加,但均较小,最大吸收率分别为8.74%、8.15%、8.77%,组间无统计学差异;在0~180 min内,低、中、高浓度QS持续增加,最大值分别为32.00、63.49、115.29 μg/cm2,45~180 min高浓度组显著大于低浓度组(P < 0.01)。结论 金莲花中的总黄酮不易被机体吸收,其吸收特征与其单体化合物一致。
[Key word]
[Abstract]
Objective To investigate the absorption properties of the total flavonoids from the flowers of Trollius chinensis for the purpose of the evaluation of the main effective components of these flowers and their further development and utilization. Methods The everted gut sac model was established, and the total flavonoids in Tyrode solution of 2, 6, and 10 mg/mL were given in the bath. Sample of 100 μL were taken from the intestinal sac 15, 30, 45, 60, 90, 120, 150, and 180 min after receiving, and the same volume blank Tyrode solution was added. The sample was processed as a test solution. Using orientin as control sample, the content of total flavonoids in the samples was determined by using microplate reader at 341 nm, and cumulative absorption quantities of the total flavonoids per unit area (QS) and the drug absorption rate (V) were calculated. Results Given the total flavone solution of low, middle and high concentration in the bath, V of intestinal sac increased gradually with the extension of time, and the maximum absorption rate were 8.74%, 8.15%, 8.77%, respectively, but there were no significant differences among groups. During the period of 0 to 180 min, QS of low, medium and high concentration increased sustainably with the maximum values of 32.00, 63.49, and 115.29 μg/cm2, respectively. During the period of 45 to 180 min, QS of high concentration group was significantly higher than that in the low concentration group (P < 0.01). Conclusion The total flavonoids from the flowers of T. chinensis are not easily absorbed by the organism, and their absorption properties are consistent with those of the monomeric compounds.
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[基金项目]
北京市自然基金面上项目(7172129)