目的 比较研究过氧化氢和氢醌两种化学脱色法制备白癜风豚鼠模型，发现存在的问题，并分析影响因素。方法 豚鼠随机分成模型组和对照组，每组12只，雌雄各半。采用3种方式制备白癜风模型：5%过氧化氢溶液局部皮肤涂抺、5%过氧化氢溶液局部皮肤封闭透皮、5%氢醌溶液局部皮肤涂抺，对照组平行给予生理盐水，连续40~50 d。肉眼观察皮肤表面局部色素脱失现象；取白斑区皮肤切片，硫酸亚铁Lillie法染色，光镜下观察皮肤组织形态和黑色素分布情况；Advanced 3.0图像分析软件测量表皮黑色素照片的平均积分光密度。结果 采用5%过氧化氢溶液两种方式造模，豚鼠均未出现皮肤色素脱失，白癜风模型未成立；采用5%氢醌溶液涂抺后，局部皮肤出现灰白色斑片状色素脱失，基本达到模型的外观要求；经组织病理学半定量或定量分析，与对照组比较，表皮含黑色素毛囊比例、黑色素分布评分、表皮平均积分光密度均显著降低（P<0.05、0.01）。结论 过氧化氢通过破坏黑色素结构脱色，对其生成影响不大，模型较难成立；氢醌为酪氨酸酶抑制剂，可减少黑色素生成，更易形成白癜风模型。

Objective To compare two chemical decolorization methods building vitiligo guinea pigs model, which were prepared by hydrogen peroxide and hydroquinone, and to discovery some existing problems and analyze the influencing factors. Methods Guinea pigs randomly divided into model and control group with 12 in each group, male and female in half. Using three ways to build vitiligo model:5% hydrogen peroxide topical skin smear, 5% hydrogen peroxide closed treatment and 5% hydroquinone solution topical skin smear. Guinea pigs in the model group were given equal volume of normal saline for consecutive 40~50 d. Local skin pigment depigmentation phenomenon was observed by naked eye. Skin sections from the white spot area were taken for ferrous sulfate Lillie staining to observe the morphology and distribution of melanin in skin tissue under light microscope. The average integral optical density of melanin photos of epidermis was measured by advanced 3.0 image analysis software. Results Using 5% hydrogen peroxide solution of two types building method, experimental animals did not appear skin depigmentation, and vitiligo model was not established; After coating with 5% hydroquinone liquid, local skin appeared gray spots flake, basically meet the requirements of the appearance of the model. In the semi-quantitative or quantitative analysis of histopathology, the melanin distribution in the skin epidermis, melanin score and the mean value of integral optical density of epidermis were significantly decreased compared with the normal control group (P < 0.01 or 0.05). Conclusion Hydrogen peroxide is a strong oxidizer, which can destroy the structure of melanin, but has little decoloring effect to set up the vitiligo model. Hydroquinone, as a tyrosinase inhibitor, can reduce the generation of melanin and are more likely to form the model of vitiligo.