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[摘要]
目的 探讨姜黄素对小鼠皮肤移植排斥反应的免疫抑制作用。方法 BALB/c小鼠作为供体,C57BL/6小鼠作为受体,建立背-背皮肤移植模型。术后分为假手术组、模型组、姜黄素(50 mg/kg)组、环孢素A (10 mg/kg)组、姜黄素+环孢素A (50 mg/kg+5 mg/kg)组,每组10只,每天1次,ip给药10 d。记录各只小鼠移植皮片的存活天数,术后第4、8天采用ELISA法检测血浆白细胞介素-2(IL-2)浓度。结果 姜黄素组、环孢素A组、姜黄素+环孢素A组的小鼠移植皮片存活时间分别为14.77、16.81、19.96 d,与模型组(12.10 d)比较差异显著(P<0.01);姜黄素+环孢素A组的小鼠移植皮片存活时间与姜黄素或环孢素A组比较显著延长(P<0.05、0.01);姜黄素组、环孢素A组、姜黄素+环孢素A组术后第4天血浆IL-2水平分别为3.68、2.05、2.70 ng/mL,与模型组(4.76 ng/mL)比较差异显著(P<0.05、0.01);姜黄素组、环孢素A组、姜黄素+环孢素A组术后第8天血浆IL-2水平分别为4.06、2.11、2.95 ng/mL,与模型组(5.85 ng/mL)比较差异显著(P<0.01)。结论 姜黄素具有抑制皮肤移植小鼠免疫排斥反应的作用。
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[Abstract]
Objective To investigate the immunosuppressive effect of curcumin on xenogenic skin transplantation in mice. Methods The skin transplantation model was established by an operation, tergal skin flaps from BALB/c donor mice transplanted to the back of C57BL/6 recipient mice. Then recipient mice were divided into five groups at random, namely sham, model, curcumin (50 mg/kg), Cyclosporin A (10 mg/kg) and curcumin+Cyclosporin A (50 mg/kg+5 mg/kg). All mice were ip administered once daily for 10 d. The survival days of skin graft were recorded in all groups. The interleukin-2 (IL-2) levels in plasma of all mice were determined by ELISA 4 and 8 d after the operation, respectively. Results The mean survival time of skin graft in curcumin, Cyclosporin A and curcumin+Cyclosporin A groups were 14.77, 16.81 and 19.96 d, respectively, which showed significant differences comparing with 12.10 d of model group (P<0.01). Combination of curcumin and Cyclosporin A administration showed a longer mean survival days than curcumin or Cyclosporin A group (P<0.05 or 0.01). The IL-2 levels in plasma of mice in curcumin, Cyclosporin A and curcumin+Cyclosporin A groups on postoperative day 4 were 3.68, 2.05 and 2.70 ng/mL, respectively, which were significantly reduced than 4.76 ng/mL of model group (P<0.05 or 0.01). The IL-2 levels in plasma of mice in curcumin, Cyclosporin A and combination of curcumin and Cyclosporin A groups on postoperative day 8 were 4.06, 2.11 and 2.95 ng/mL, respectively, which were significantly reduced than 5.85 ng/mL of model group (P<0.01). Conclusion Curcumin may have a good immunosuppressive effect on mice with xenogenic skin transplantation.
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