目的 建立同时测定芍药苷、阿魏酸、芸香柚皮苷、柚皮苷、新橙皮苷、甘草酸含量的超高效液相色谱（UPLC）法，研究柴胡疏肝散水提物的肠吸收特性。方法 采用大鼠在体单向肠灌流和外翻肠囊实验模型，应用UPLC法测定柴胡疏肝散水提物中指标性成分（芍药苷、阿魏酸、芸香柚皮苷、柚皮苷、新橙皮苷和甘草酸）在不同时间点、不同部位肠道吸收量，计算吸收动力学参数，考察其肠吸收特征。结果 在体单向肠灌流模型结果表明，芍药苷、芸香柚皮苷、新橙皮苷、柚皮苷和甘草酸均为中等程度吸收的化学成分，而阿魏酸为完全吸收；外翻肠囊模型结果表明，芍药苷在空肠部位，阿魏酸、柚皮苷和新橙皮苷在十二指肠部位，芸香柚皮苷在十二指肠和空肠部位的吸收最佳，而甘草酸在各肠段的吸收无显著性差异。结论 肠道对柴胡疏肝散中6种指标性成分均有吸收，阿魏酸较其他5种指标性成分更易透过肠壁进入血液循环；不同肠段对6种指标性成分的吸收具有选择性。

Objective To evaluate the intestinal absorption of main components (paeoniflorin, ferulic acid, narirutin, naringin, neohesperidin, and glycyrrhizic acid) in aqueous extract of Chaihu Shugan San (CSS). Methods UPLC method was established to simultaneously determine the concentrations of the six components in the test samples of everted gut sacs and in situ single pass intestinal perfusion (SPIP) models. Absorption kinetics parameters were calculated for describing their absorption characteristics. Results In SPIP model, the results indicated that ferulic acid was the well-absorbed ingredient in whole small intestine, while other ingredients presented moderate or poor absorption. In everted gut sacs model, paeoniflorin in jejunum, ferulic acid, naringin, and neohesperidin in duodenum, narirutin in duodenum and jejunum, had the best absorption, while there was no significant difference in absorption of glycyrrhizic acid in the intestine. Conclusion In vivo model indicated that the main constituents in CSS could be absorbed in intestinal wall of rat, ferulic acid could be much more easily penetrated intestinal wall into the blood circulation than the other five components. Ex vivo model could further articulate that six index components could be absorbed selectively in different intestinal segments.

国家自然科学基金项目（NO.81673584）