目的 建立一测多评法检测华佗豆药材中4种生物碱的量。方法 以华佗豆碱乙为内标，建立华佗豆碱甲、碱丙、碱丁的相对校正因子，在考察相对校正因子的重现性后，进一步采用外标法与一测多评法所计算出的成分含量进行比较。结果 6批华佗豆药材中，各成分采用相对因子计算的量与采用外标法计算的结果无显著差异。结论 一测多评法操作简单、结果准确、可靠，可用于华佗豆4种生物碱的测定。

Objective To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining four compounds in Calonyction muricatum.Method An HPLC method was developed as QAMS to determine ipalbidine, ipalbidinium, and ipalbinium in C. muricatum, using ipalbine as the intermal reference substance, the relative correction factor (RCF) of the three components was determined by HPLC with good reproducibility. Their contents in six batches of samples were determined by both external standard method and QAMS.Result No significant differences were found in the quantitative results of three alkaloid compounds in six batches of C. muricatum determined by external standard method and QAMS.Conclusion It is feasible and suitable to evaluate the quality of curcuma aromatic by QAMS.