目的 通过观察元胡止痛滴丸及其组分对缩宫素痛经模型的影响，并与临床上同类药月月舒对比，探讨其配伍的合理性及比较优势。方法 通过ip缩宫素诱发大鼠痛经模型，观察元胡止痛滴丸及其组成成分延胡索浸膏、白芷浸膏对扭体反应潜伏期及次数的影响；ELISA法检测与疼痛相关血浆中5-羟色胺（5-HT）、去甲肾上腺素（NA）、β-内啡肽（β-EP）、前列腺素F_2α（PGF_2α）、前列腺素E₂（PGE₂）水平，脑组织中多巴胺（DA）水平；金氏概率相加法计算Q值，根据Q值是否大于1判断元胡止痛滴丸的效果是否优于组分单用。结果 与模型组比较，元胡止痛滴丸、延胡索浸膏和白芷浸膏能够显著减少扭体次数、延长扭体反应的潜伏期（P<0.05、0.01、0.001），Q值均大于1；与月月舒比较，元胡止痛滴丸作用显著增强（P<0.05、0.01）。与模型组比较，元胡止痛滴丸和延胡索浸膏可显著升高β-EP、PGE₂水平（P<0.05、0.01），显著降低5-HT、NA、DA、PGF_2α水平及PGF_2α/PGE₂比值（P<0.05、0.01、0.001）；白芷浸膏可显著降低5-HT、DA水平（P<0.05、0.01）；β-EP、NA、DA和PGE2的Q值均大于1；与月月舒比较，元胡止痛滴丸可显著升高β-EP、PGE₂水平，显著降低5-HT、PGF_2α水平及PGF_2α/PGE₂比值（P<0.05、0.01）。结论 元胡止痛滴丸对痛经模型具有显著的镇痛作用，作用强度优于组分单用，配伍具有合理性，与月月舒比较具有起效早、作用强的特点。

Objective To observe compatibility rationality and comparative advantage of analgesic effect of Yuanhu Zhitong Dropping Pills (YZDP) compared with its ingredient and Yueyueshu through dysmenorrhea model induced by oxytocin.Methods Using dysmenorrhea model induced by ip injection of oxytocin to observe the effect of YZDP, Corydalis extract and Angelica dahurica extract on the latency and times of writhing response, and ELISA method was used to test the level of factors associated with pain in plasma, including 5-serotonin (5-HT), norepinephrine (NA), β-endorphin (β-EP), prostaglandin F_2α (PGF_2α) and prostaglandin E₂ (PGE₂), and level of dopamine (DA) in brain tissue. Q value was calculated by prime probability addition method, and according to whether the Q value was greater than one to determine whether the effects of YZDP was better than that of single component.Results Compared with model group, YZDP, Corydalis extract and Angelica dahurica extract could significantly prolong the latency of writhing response, decrease writhing times (P<0.05, 0.01 and 0.001), and the Q values were greater than one. Compared with Yueyueshu, effect of Yuanhuzhitong dropping pills was significantly increased (P<0.05 and 0.01). YZDP and Corydalis extract could increase the level of β-EP as well as PGE₂, and decrease the level of 5-HT, NA, DA, PGF_2α and the ratio of PGF_2α to PGE₂ obviously in dysmenorrhea model rats (P<0.05, 0.01 and 0.001). A. dahurica extract can significantly reduce 5-HT and DA level (P<0.05, 0.01). Q values of β-EP, NA, DA and PGE₂ were greater than one. Compared with Yueyueshu, YZDP increased the levels of β-EP and PGE₂, and decreased the levels of 5-HT, PGF_2α and PGF_2α/PGE₂ significantly (P<0.05 and 0.01).Conclusion YZDP has significant analgesic effect on dysmenorrhea model and has compatibility rationality because of better analgesic effect than ingredient use only. Compared with Yueyueshu, YZDP has the characteristic of working early and better analgesic effect.

国家科技部“重大新药创制”科技重大专项（No2015ZX09501004-002-003）