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[摘要]
目的 探讨莪术油对直肠癌SW1463细胞株增殖、凋亡及相关蛋白Caspase-3、Bax、Bcl-2表达的影响。方法 水蒸气蒸馏法提取黔产莪术挥发油,配制成40、80、120、160、200、240、280 mg/L浓度梯度,干预SW1463细胞24、48、72 h,MTT法检测莪术油对SW1463细胞的增殖抑制率;Giemsa染色法观察莪术油对SW1463细胞凋亡形态的影响;蛋白免疫印迹法(Western blotting)检测Capase-3、Bax与Bcl-2蛋白表达。结果 莪术油对SW1463细胞的增殖有明显抑制作用,并呈现时间-剂量相关性,24、48、72 h的半数抑制浓度(IC50)分别为144.33、134.11、120.04 mg/L;Giemsa染色可见细胞明显的凋亡形态学特征;莪术油干预SW1463细胞24 h后,与对照组比较,Caspase-3、Bax蛋白表达显著上调、Bcl-2蛋白表达显著下调(P<0.05)。结论 莪术油能明显抑制SW1463细胞增殖,诱导细胞凋亡,其机制可能与上调Caspase-3和Bax蛋白表达、下调Bcl-2蛋白表达相关。
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[Abstract]
Objective To explore the effects of zedoary turmeric oil on proliferation and apoptosis of SW1463 cell line and the expression of Caspase-3, Bax and Bcl-2.Methods Volatile oil from Curcumae Rhizoma in Guizhou was extract by steam distillation, which was used to intervene SW1463 cells for 24, 48 and 72 h at concentration of 40, 80, 120, 160, 200, 240 and 280 mg/mL. MTT method was used to detect the inhibitory rate of zedoary turmeric oil on SW1463 cell proliferation. Effects of different concentrations of zedoary oil on apoptosis of SW1463 cells were observed by Giemsa staining. Western blotting was used to detect Capase-3, Bax and Bcl-2 protein expression.Results Zedoary turmeric oil inhibited the proliferation of SW1463 cells and showed a time dose correlation, and half maximal inhibitory concentration (IC50) of 24, 48 and 72 h was 144.33, 134.11 and 120.04 mg/L, respectively. Giemsa staining showed obvious morphological characteristics of apoptotic cells. Western blotting results showed that compared with control group, the expression of Caspase-3 and Bax in cells treated with zedoary turmeric oil for 24 h were significantly up-regulated (P<0.05), and the expression of Bcl-2 protein was significantly down-regulated (P<0.05).Conclusion Zedoary turmeric oil can obviously inhibit the proliferation of SW1463 cells and induce apoptosis, which may be related to the up-regulation of Caspase-3 and Bax protein expression and down-regulation of Bcl-2 protein expression.
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[基金项目]
贵州省厅校联合项目[黔科合LG字(2011)006号];贵州省社会发展项目[黔科合SY(2008)3027号]