目的 建立酸枣叶药材的HPLC指纹图谱，为其质量标准的研究提供依据。方法 采用Kinetex C₁₈色谱柱（100 mm×2.1 mm，2.6 μm）；流动相为乙腈-0.1%磷酸酸水溶液，梯度洗脱；体积流量为300 μL/min；柱温30℃，检测波长225 nm，进样量10 μL，对12批酸枣叶药材进行了指纹图谱研究，采用中药色谱指纹图谱相似度评价系统（2012版）软件进行分析。结果 12批酸枣叶的HPLC指纹图谱有13个共有峰，其中1个共有峰得到确认，相似度均＞0.85。结论 该方法准确可靠，重复性好，为更好地控制酸枣叶内在质量提供科学依据。

Objective To establish a specific HPLC method and a standard fingerprint for quality control of Ziziphi Spinosae Folium. Methods The samples were separated on a Kinetex C₁₈ column (100 mm ×2.1mm, 2.6 μm) by gradient elution at the flow rate of 300 μL/min using acetonitrile and 0.1% aqueous formic acid as the mobile phase. The column temperature was 30℃. The detection wavelength was 225 nm and the sample size was 10 μL. The fingerprint evaluation software (2012 edition) for Chinese materia medica (CMM) was used to evaluate the similarity of the 12 batches of samples. Results There were 13 characteristic peaks identified in the characteristic spectra of Ziziphi Spinosae Folium samples.Peak 6 was Rutin.The similarities of 13 batches of Ziziphi Spinosae Folium samples were proved to be higher than 0.850. Conclusion The method is available with a good reproducibility and accuraty which can control the quality standards effectively.

河北省自然基金研究项目（H2015423026）；河北省高等学校科学技术研究项目（ZD2015001）；河北省中医药管理局科研计划项目（2017013）