[关键词]
[摘要]
目的 研究不同产地枇杷叶醇提物对抗炎靶标磷酸二酯酶4(PDE4)的抑制作用。方法 采用体积分数95%乙醇回流提取法提取药材,以标记液体闪烁计数法检测提取物对PDE4的D2亚型靶标(PDE4D2)的抑制活性。结果 测试体系的药物终质量浓度为5 mg/L时,20个样品中9个测试样品的抑制率在80%以下;11个样品的抑制率大于80%,其中8个样品大于90%。结论 不同地枇杷叶醇提物抑制PDE4靶标的活性存在明显差异。
[Key word]
[Abstract]
Objective To investigate the inhibition of Eriobotryae Folium from twenty different districts towards phosphodiesterase 4(PDE4) in vitro. Methods The Eriobotryae Folium were extracted with 95% ethanol reflux and the inhibition rates against PDE4D2 were carried out by liquid scintillation counting method. Results All the samples exhibited inhibitory activities towards PDE4 at 5 mg/L. Among them, nine samples were of the inhibition rate less than 80%, eleven samples were of more than 80% inhibition and eight samples were of more than 90% inhibition. Conclusion The Eriobotryae Folium shows significantly different inhibitory activities towards PED4.
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[基金项目]
国家自然科学基金项目(21103234,81373258);广东自然科学基金团队项目(S2011030003190);广州市科技计划项目(201607010293,2014J4100165);国家公益性行业专项(201407002)