[关键词]
[摘要]
目的探究一种更加准确、合理且对仪器影响较小的电感耦合等离子体质谱(ICP-MS)重金属检测方法的在线内标物浓度。方法选择标准曲线范围内10、50、200、500、1 000 μg/L的质量浓度水平作为内标溶液在线加入,测定栀子药材中V、Cr、Mn、Fe、Ni、Cu、Zn、As、Cd、Pb和Hg元素。结果低浓度水平内标不足以校正仪器波动,对测定结果影响较大;高浓度内标溶液易造成污染,在校正仪器的同时容易掩盖信号,影响进样前调谐;500 μg/L内标既能使待测元素有稳定的响应值,又不会过于抑制信号。结论内标浓度的选择既要兼顾标准曲线进样浓度,又要避免浓度过高造成的信号掩盖,500 μg/L的内标浓度能够有效补偿中药材金属元素测定过程的基体效应,测定结果相对准确。
[Key word]
[Abstract]
Objective To provide a proper concentration of Internal Standard Solution by comparing five different degrees to the simultaneous analysis of 10 metals including V, Cr, Mn, Fe, Ni, Cu, As, Cd, Pb and Hg in Cardenia jasminoides. Method An Inductively Coupled Plasma-Mass Spectrometry method with Microwave-Assisted Digestion was developed with 10, 50, 200, 500, 1 000 μg/L of Ge, In, Bi as internal standard. Result The degree of 500 μg/L concentration of internal stand solution can both correct the matrix effect and alleviate the contamination as the 1 000 μg/L. Conclusion With excellent detection limits, high sensitivity and wide linear range, the 500 μg/L internal standard may serve as reference in the determination of elements in other traditional Chinese medicine (TCM).
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[基金项目]