目的 观察如意珍宝丸对斑马鱼神经损伤的保护与促再生作用。方法 用吗替麦考酚酯诱导斑马鱼中枢神经损伤模型；用乙醇诱导建立斑马鱼外周运动神经损伤及轴索损伤模型；用溴化乙锭诱导建立斑马鱼髓鞘损伤模型。分别在荧光显微镜下观察高、中、低质量浓度（10.0、33.3和100.0 μg/mL）的如意珍宝丸对模型斑马鱼的中枢神经、轴索荧光强度、外周运动神经长度以及髓鞘荧光强度变化的影响，利用NIDS-Element's软件分别进行图像分析，计算如意珍宝丸对斑马鱼中枢神经、轴索的保护率、对外周运动神经及髓鞘再生的促进率。结果 质量浓度分别为10.0、33.3和100.0 μg/mL的如意珍宝丸对斑马鱼中枢神经损伤保护率分别为-2%、24%和50%（P<0.001）；轴索损伤的保护率分别为3%、29%和48%（P<0.05）；外周神经再生促进率分别为44%（P < 0.05）、49%（P < 0.01）和93%（P < 0.001）；髓鞘再生促进率分别为36%（P < 0.01）、37%（P < 0.001）和41%（P < 0.001）。结论 如意珍宝丸对斑马鱼中枢神经及轴索损伤具有保护作用，对斑马鱼外周运动神经损伤及髓鞘损伤具有促再生作用。

Objective To observe the protective and regeneration-promoting effects of Ruyi Zhenbao Pill (RZP) on nerve injury in zebrafish. Methods The zebrafish model of central nervous injury was induced by mycophenolate mofetil, the model of peripheral motor nerve and axonal injury was induced by ethanol, and the model of myelin damage was induced by ethidium bromide. The variations of central nerve, axon and myelin sheath fluorescence intensity, and peripheral motor nerve length in zebrafish, which exposed to the different concentration (10.0, 33.3, and 100.0 μg/mL) of RZP, were observed with fluorescence microscope. The effective protection rates of RZP on zebrafish central nerve and axone, and the regeneration-promoting effect on peripheral motor nerve and myelin sheath were analyzed and calculated with the image processing software NIDS-Element's. Results In 10.0, 33.3, and 100.0 μg/mL RZP groups, the zebrafish central nervous injury protective rates were 2%, 24%, and 50% (P<0.001), respectively, the peripheral nerve regeneration promoting rates were 44% (P < 0.05), 49% (P < 0.01), and 93% (P < 0.001), the axonal injury recovery rates were 3%, 29% and 48% (P<0.05), and the myelin sheath regeneration promoting rates were 36% (P < 0.01), 37% (P < 0.001), and 41% (P < 0.001), compared with model group. Conclusions RZP could not only protect the central nervous and axonal injury, but also promote the regeneration of peripheral nerve and myelin sheath in zebrafish.

浙江省科技重大专项（2014C03009）