目的 建立测定金线莲中阿魏酸的定量方法，为金线莲及其相关产品的质量控制提供科学的方法。方法 采用RP-HPLC法，选用Lanbo-Kromasil-C18色谱柱；以甲醇-1%乙酸溶液（32∶68）为流动相；检测波长321 nm，体积流量1.0 mL/min，柱温为25℃。结果 阿魏酸在36~1 080 ng与峰面积呈良好的线性关系（r=0.999 9）。平均加样回收率为99.7%，RSD=2.5%。结论 本法简便、快速、准确、重复性好，可作为金线莲质量控制的方法。

Objective To establish a method for determination of the content of ferulic acid in Anoectochilus roxburghii. and to provide a scientific method for quality control of A. roxburghii and related products. Methods The conditions were as follows:reverse phase HPLC and Lanbo-Kromasil-C18 column, methanol-1% acetic acid solution (32:68) as mobile phase, detection wavelength of 321 nm, the volume flow rate of 1 mL/min, and column temperature of 25℃. Results Ferulic acid showed good linear relationship in the range of 36-1 080 ng (r=0.999 9). The average recovery was 99.7%, and the average rate of recovery was RSD=2.5%. Conclusion This method is simple, rapid, accurate, and reproducible, and can be used as an ideal method for the quality control of A. roxburghii.