目的 观察补肾调冲方对卵巢早衰模型大鼠血清促卵泡刺激素（FSH）、促黄体生成素（LH）、抗缪勒管激素（AMH）水平及卵巢组织肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）蛋白表达的影响。方法 60只SD雌性大鼠随机分为6组：对照组，模型组，补肾调冲方低、中、高剂量（以生药计24、12、6 g/kg）组，结合雌激素片（阳性药）组，每组10只。除对照组外，其余各组均ig雷公藤多苷片造模14 d，造模结束24 h后，给予相应药物治疗36 d。给药结束后，酶联免疫吸附（Elisa）法检测大鼠血清FSH、LH、AMH水平，免疫组化法检测卵巢组织TNF-α、IFN-γ蛋白表达。结果 与对照组比较，模型组血清FSH、LH水平、卵巢TNF-α、IFN-γ蛋白表达均显著升高，AMH水平显著降低（P<0.05）；与模型组比较，各给药组FSH、LH水平、卵巢TNF-α、IFN-γ蛋白表达均显著降低，AMH水平显著升高（P<0.01）。结论 补肾调冲方可提高卵巢早衰大鼠卵巢储备能力，抑制卵泡封闭及黄体细胞凋亡，对卵巢早衰可能有治疗作用。

Objective To observe the effect of Bushen Tiaochong Prescription (BTP) on sex hormone level and TNF-α, IFN-γ protein expression in rats with premature ovarian failure. Methods Totally 60 SD female rats were randomly divided into six groups with 10 rats in each group:control group, model group, BTP low, medium, and high dose (24, 12 and 6 g/kg in crude drugs) groups, and Conjugated Estrogens Tablets (positive drug) group. Rats were ig administered with tripterygium glycosides for 14 d to make model of premature ovarian failure except for control group. Rats were treated with corresponding medicines for 36 d 24 h after that. Then the enzyme linked immunosorbent assay (Elisa) method was used to detect the levels of AMH, LH, and FSH in serum, and the expression of TNF-α and IFN-γ in ovarian tissue was detected by immunohistochemistry. Results Compared with control group, the levels of serum FSH, LH and ovarian TNF-α, IFN-γ protein expression of model group was increased significantly, and the level of serum AMH was significantly decreased (P<0.05); Compared with model group, levels of FSH, LH, TNF-α, and IFN-γ of BTP groups and Conjugated Estrogens Tablets group were significantly decreased, AMH level was significantly increased (P<0.01) Conclusion BTP can improve the ovarian reserve, inhibit follicle blocking and apoptosis of corpus lutein cells of the premature ovarian rats, which suggests that BTP may have a therapeutic effect on premature ovarian disease.

河北省省级重大医学科研项目（zyzd2013018）；河北省政府资助临床医学优秀人才培养项目（2014571024）；国家中医药管理局高慧全国名老中医药专家传承工作室建设项目（2015048157）