目的 研究黑木耳多糖（AAP）和红松多酚（PKP）协同对⁶⁰Coγ辐射致小鼠免疫损伤的修复作用。方法 昆明小鼠除对照组外⁶⁰Coγ射线一次性全身均匀照射，总吸收剂量为4.0 Gy，吸收剂量率为1.0 Gy/min。辐射24 h后分别设AAP组，PKP组，AP（AAP与PKP协同）组及灵芝多糖（GLP）组，分别ig 75.0、25.0、37.5+12.5、75.0 mg/kg（以多糖或多酚水平计）相应药物，同时对照组和模型组小鼠ig等体积生理盐水。连续喂养30 d后空腹24 h处死，测定小鼠的胸腺、肝脏、脾脏、心脏、肾脏和脑的器官指数；瑞士－吉姆萨染液染色测定正常外周血白细胞数量；碳廓清实验检测吞噬指数（α）；MTT法检测脾淋巴细胞刺激指数（SI）。结果 辐射后给药PKP、AAP、AP、TP和GLP都会一定程度升高小鼠肝脏、脾脏、心脏和胸腺的器官指数，增加正常外周血白细胞数量，升高α和SI，其中AP组对辐射后修复效果较好。结论 比较AAP、PKP、TP及GLP，AP显著的结合了AAP和PKP的优点，从特异性免疫和非特异性免疫对辐射后小鼠进行了最大限度的修复。

Objective To study the effect of polysaccharides from Auricularia auricula (AAP) and polyphenols from Pinus Koraiensis (PKP) combination (AP) on recoveries of immunologic injury induced by ⁶⁰Coγ radiation in mice. Methods The model group and five experimental groups were irradiated once with ⁶⁰Coγ ray. AAP, PKP, AP, and Ganoderma lucidum polysaccharides (GLP) had been given to mice by gavage for 30 d at doses of 75.0, 25.0, 37.5+12.5, and 75.0 mg/kg (calculated with polysaccharide or polyphenol levels), respectively. The control and model groups were given normal saline by gavage during that time. After 30 d, the organ indexes of thymus, liver, spleen, heart, kidney, and brain, normal white blood cell counts, phagocytosis indexes (α), and stimulation indexes (SI) of mice were determined. Results AAP, PKP, AP, and GLP could significantly increase the organ indexes of thymus, liver, spleen, heart; normal white blood cell counts, α, and SI. Above all, effect of AP on immune recovery was the best. Conclusion Compared to AAP, PKP, and GLP, AP could combine the advantages of AAP and PKP, preformed the best effect of specific immunity function and non-specific immunity function.

国家自然科学基金（31170510）资助项目；中央高校基本科研业务费专项（DL11BA18）