[关键词]
[摘要]
目的 建立25-OH-PPT、25-OCH3-PPT、PPT、PT同步检测的方法;从人参根皂苷水解产物中筛选发现具有降血糖活性的三醇组皂苷元。方法 采用HPLC-ELSD法测定25-OH-PPT、25-OCH3-PPT、PPT、PT;采用体外实验测定4种皂苷元对α-葡萄糖苷酶的抑制作用。结果 HPLC-ELSD测定结果表明,25-OH-PPT、25-OCH3-PPT、PPT、PT在0.05~1.0 mg具有良好的线性关系,回收率分别为95.0%、98.4%、103.3%和96.0%,RSD分别为1.05%(n=4)、1.87%(n=4)、2.15%(n=4)和1.15%(n=4),质量分数分别为8.07%、4.55%、1.46%和4.53%。4种皂苷元对α-葡萄糖苷酶呈现出不同强度的抑制活性,25-OH-PPT抑制活性强于阿卡波糖。结论 4种皂苷元降血糖活性强弱顺序为25-OH-PPT > 25-OCH3-PPT > PPT > PT。HPLC/ELSD测定方法简单、快速、有效。
[Key word]
[Abstract]
Objective To determine the inhibitory activity of rare ginsenosides on α-glucosidase, and to select hypoglycemic compounds from ginsenosides. Meanwhile, to establish a method to determinate the contents of ginsenoside 25-OH-PPT, 25-OCH3-PPT, PPT, and PT in hydrolysis products of total saponins in ginseng roots. Results The inhibitory activity of ginsenosides on α-glucosidase revealed that the inhibitory activities of 25-OH PPT, 25-OCH3-PPT, PPT, and PT were better than that of positive drug acarbose while PT was worse than acarbose. Meanwhile, HPLC-ELSD results revealed that 25-OH PPT, 25-OCH3-PPT, PPT, and PT showed good linear relationship in the region of (0.05-1.0) mg/mL. The recoveries were 95.0%, 98.4%, 103.3%, and 96.0%, respectively, RSD values were 1.05% (n=4), 1.87% (n=4), 2.15% (n=4), and 1.15% (n=4), respectively, and the content are 8.07%, 4.55%, 1.46%, and 4.53% respectively. Conclusion The order of their hypoglycemic acitivity is 25-OH-PPT > 25-OCH3-PPT > PPT > PT. Assay method of HPLC/ELSD is simple, fast, and effective.
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[基金项目]
中药标准提取物的工业化色谱制备平台建设(2009ZX09301-012-105B);天然活性成分的工业化色谱制备公共服务平台(2011412004-7)