目的 比较半夏白术天麻汤不同提取方法对大鼠血清中单胺类递质水平的影响。方法 原发性痰湿壅盛型高血压大鼠，随机分为模型组、半仿生-酶法提取液（SBEE）组、水提液（SBE）组、醇提液（WE）组、半仿生提取液（AE）组，4种提取液均过中空纤维膜截留相对分子质量≤1 000。模型组饮用去离子水，不限量；SBEE组、SBE组、WE组、AE组分别ig相应提取液，每天1 mL（2.83 g/mL）；正常血压大鼠8只作为对照组。喂养4周后，采用高效液相色谱-电化学（HPLC-ECD）法测定血清中单胺递质去甲肾上腺素（NE）、5-羟色胺（5-HT）水平。结果 4种提取液均可降低高血压大鼠NE和5-HT水平，与模型组比较差异显著（P<0.01），对NE影响顺序是：SBEE > SBE > AE > WE，对5-HT影响顺序是：SBEE > SBE > WE > AE。结论 半夏白术天麻汤4种方法提取均可降低高血压大鼠单胺递质水平，且加入酶解过程的SBEE提取方法效果最好。

Objective To investigate the effect of four extracting solutions in Banxia Baizhu Tianma Decoction (BBTD) with relative molecular mass≤1 000 on levels of monoamine neurotransmitters in rat plasma. Method Primary phlegm dampness type hypertensive rats were randomly divided into model group, SBEE group, SBE group, WE group, and AE group, respectively, by ig administration of corresponding semi-bionic enzymetic extract, water extract, alcohol extract, and semi-bionic extraction liquid, 1 mL (2.83 g/mL) daily. Rats with normal blood pressure were used as control group. After four weeks, the norepinephrine (NE) and 5-serotonin (5-HT) content of serum were determined by HPLC method. Results Compared with model group, totally four extracting solutions of BBTD could reduce NE and 5-HT levels significantly in hypertension rats (P<0.01), order of the effect was SBEE > SBE > AE > WE and SBEE > SBE > WE > AE, respectively. Conclusion Totally four extracting solutions of BBD can reduce monoamine transmitters in hypertension rats and SBEE extraction is better.

浙江省自然科学基金（No. LY16H270018）；国家科技部国际合作项目（2007DFA31180）