[关键词]
[摘要]
目的 研究金丝桃苷(Hyp)对急性炎症疼痛模型小鼠的抗炎镇痛作用。方法 昆明种小鼠随机分为对照组,Hyp低、中、高剂量(50、100、200 mg/kg)组及氢化可的松(阳性药,20 mg/kg)组,sc给药,每天给药1次,连续给药5 d后,建立二甲苯所致小鼠耳廓肿胀和醋酸所致小鼠腹腔毛细血管通透性增高的急性炎症模型,观察Hyp对小鼠急性炎症的抗炎作用;阳性药设置为吗啡(1 mg/kg),其余分组及给药方式同急性炎症实验,连续给药5 d后,建立小鼠醋酸扭体法和热甩尾法模型,观察Hyp对急性疼痛的镇痛作用。结果 100、200 mg/kg的Hyp能够抑制二甲苯所致小鼠耳廓肿胀、降低急性炎症引起的毛细血管通透性增高、减少醋酸扭体次数、提高小鼠热刺激痛阈值,与对照组比较差异显著(P<0.05、0.01)。结论 Hyp对小鼠急性炎症疼痛具有抑制作用。
[Key word]
[Abstract]
Objective To investigate the anti-inflammatory and analgesic effects of hyperin (Hyp) in mice with acute inflammatory pain. Methods Kunming mice were randomly divided into five groups: control group, Hyp low, medium and high dose (50, 100, and 200 mg/kg) groups, and hydrocortisone (positive drug, 20 mg/kg) group, sc relative drug once daily for 5 d. The anti-inflammatory actions of Hyp were tested by evaluating auricle edema levels induced by xylene and blood capillary leakage of abdominal cavity in mice. The positive drug was set to morphine (1 mg/kg), drug administration of other groups was the same as acute inflammation test. After 5 d, acetic acid writhing and thermal tail-flick tests were used to measure the acute analgesic effects. Results Both 100 and 200 mg/kg Hyp inhibited auricle edema levels, reduced blood capillary leakage, decreased the number of writhing, and increased the pain threshold of thermal stimulation. Conclusion Hyp has inhibitory effects in mice with acute inflammatory pain.
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[基金项目]
国家自然科学基金面上项目(3077263)