目的 研究益气消癥法方含药血清对EA.hy926细胞丝裂原活化蛋白激酶（MAPK）信号通路上细胞外信号调节激酶1/2（ERK1/2）表达的影响，探索其抑制子宫肌瘤血管新生的作用机制。方法 SD大鼠随机分为：对照组，益气消癥法方高、中、低剂量（生药剂量24、12、6 g/kg）组，ig给药，每天给药1次，连续给药5 d，腹主动脉取血，分离血清，灭活、过滤除菌；体外培养EA.hy926细胞，随机分为6组：对照血清组、模型组及益气消癥法方高、中、低剂量血清组和氟维司群（ICI，阳性药）组，除对照血清组外，其余组别给予雌二醇（E₂），诱导细胞增殖，制备子宫肌瘤血管模型；通过实时荧光定量PCR（qRT-PCR）及Western blotting法检测MEK2和ERK1/2基因与蛋白的表达。结果 模型组MEK2和ERK1/2基因与蛋白的表达水平明显高于对照血清组（P<0.01）；与模型组比较，ICI和益气消癥法方高剂量血清组MEK2和ERK1/2表达水平显著降低（P<0.01）。结论 益气消癥法方含药血清通过降调ERK1/2表达，阻断MAPK/ERK1/2信号转导，抑制雌激素诱导的血管内皮细胞增殖，减少子宫肌瘤血液供应。

Objective To investigate the intervention of Yiqi Xiaozheng Prescription (YXP)-contained serum on expression of extracellular-signal regulated kinase 1/2 (ERK1/2) based on mitogen-activated protein kinase (MAPK) signal pathway in EA.hy926 cells, and to investigate the mechanism of inhibiting angiogenesis in uterine leiomyoma. Methods SD rats were divided into four groups: control group; YXP low, medium, and high dose groups (24, 12, and 6 g/kg at crude drug dosage). After sc administration once daily for 5 d, blood plasma was collected by abdominal aortic method, serum was isolated, inactivated, and filtrated rid of bacterium. EA.hy926 cells cultured in vitro were divided into six groups: control serum group; model group, YXP low, medium, and high dose contained serum group, and filtration (ICI, positive drug) group. Proliferation of EA.hy926 cells was induced by 17β-estradiol (E₂) to simulate angiogenesis in uterine leiomyoma except control serum group. Real-time fluorescence quantification PCR (qRT-PCR) and Western blotting were used to detect the gene and protein expression of MEK2 and ERK1/2. Results MEK2 and ERK1/2 gene and protein expression levels of model group were significantly higher than those of control group (P<0.01); Compared with model group, MEK2 and ERK1/2 expression levels of YXP high dose contained serum group and ICI group were significantly decreased (P<0.01). Conclusion YXP-contained serum could down-regulate the expression of ERK1/2, block the MAPK/ERK1/2 signal transduction, and inhibit the proliferation of vascular endothelial cells which was induced by estrogen in order to control angiogenesis and reduce blood supply of uterine leiomyoma.

国家自然科学基金面上项目（30973768）