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[摘要]
目的 研究藏药川西獐牙菜提取物对人胃癌细胞MGC-803增殖抑制作用和细胞周期的影响。方法 MTT法测定川西獐牙菜石油醚部位、醋酸乙酯部位、正丁醇部位、水部位和甲醇总提取物部位对人胃癌细胞MGC-803的增殖抑制作用;流式细胞术检测正丁醇部位对细胞周期的影响;高效液相色谱-串联质谱(HPLC-MS/MS)法检测正丁醇部位的主要化学成分。结果 藏药川西獐牙菜各提取部位对人胃癌细胞MGC-803增殖均有抑制作用,正丁醇部位的增殖抑制效果最佳且具有明显的量效关系;用正丁醇部位处理MGC-803细胞,表现出明显的S期周期阻滞;HPLC-MS/MS检测结果表明正丁醇部位的主要化学成分为1,7,8-三羟基-3-甲氧基呫吨酮、1,8-二羟基-3,5-二甲氧基呫吨酮、1,3,6,7-四羟基呫吨酮-2-β-D-葡萄糖、2’-间羟基苯甲酰獐牙菜苷、獐牙菜苷-6’-O-葡萄糖和1-羟基-2,3,5-三甲氧基呫吨酮-1-O-[β-D-吡喃木糖(1-6)-β-D-吡喃葡萄糖]。结论 川西獐牙菜各部位对人胃癌细胞MGC-803均具有增殖抑制作用,正丁醇部位的抑制作用最强且能够通过改变细胞周期分布抑制人胃癌细胞MGC-803的生长,其中主要成分为呫吨酮类化合物。
[Key word]
[Abstract]
Objective To investigate proliferation and cell cycle of MGC-803 cancer cells treated with butanol extracts of Tibetan medicine Swertia mussotii (BESM). Methods Proliferation of human gastric cancer MGC-803 cells treated with BESM were assayed by MTT in vitro, and the change of cell cycles treated with BESM was detected by flow cytometry. The main compounds of BESM have been analyzed by HPLC-MS-MS. Results BESM could inhibit the proliferation of human gastric cancer MGC-803 cells; After treating with BESM, MGC-803 cell cycle was arrested remarkably at S phase. The main compounds from BESM were 1,7,8-trihydroxy-3-methoxyxanthone, 1,8-dihydrox-3,5-dimethoxyxanthone, 1,3,6,7-tetrahydroxyxanthone-2-β-D-glucopyranosyl, 2'-m-hydroxybenzoylsweroside, 6'-O-glucopyranosylsweroside, and 1-O-[β-D-xylopyranosyl-(1→6)-β-D-glucopyranosyl]-3,5- dimethoxyxanthone. Conclusion BESM could inhibit the growth of human gastric cancer MGC-803 cells, and can be used to regulate MGC-803 carcinoma cells proliferation by inhibiting cells in S phase, and the main component of it is xanthone.
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[基金项目]
国家自然科学基金(11372162与11432008)