目的 建立癃闭舒片中补骨脂素和异补骨脂素的定量方法。方法 对国内3个企业生产的6个批次的癃闭舒片,采用HPLC法检测,Agilent TC-C₁₈(2)色谱柱(250 mm×4.6 mm,5μm),流动相是甲醇-水(43:57),体积流量1.0 mL/min,检测波长为246 nm,柱温30℃。结果 补骨脂素回归方程为:Y=84.44X+9.994,r=0.9999(n=12)。异补骨脂素回归方程为:Y=82.434X+8.8952,r=0.9999(n=12)。补骨脂素和异补骨脂素的线性范围均在4.04~151.5μg。结论 本方法操作简便、准确、专属性强、稳定,可用于癃闭舒片中补骨脂素和异补骨脂素的含量测定。

Objective To establish a method for the content determination of psoralen and isopsoralen in Longbishu Pills (LBSP). Methods HPLC was performed on the Agilent TC-C₁₈(2) column (250 mm×4.6 mm, 5 μm) with the mobile phase of MeOH-H2O (43:57), the flow rate was 0.8 mL/min, the detection wavelength 246 nm, and the column temperature was 30℃. Results The element regression equation of psoralen was Y=84.44X+9.994, r=0.999 9 (n=12). The element regression equation of isopsoralen was Y=82.434X+8.895 2, r=0.999 9 (n=12). The concentrations of psoralen and isopsoralen were within the range of 4.04-151.5 μg. Conclusion The method is simple, reliable, and specific, with good reproducibility, and could be used to determine the content of psoralen and isopsoralen in Longbishu Pills.

辽宁省自然科学基金优秀人才培养项目(2015020698)