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[摘要]
目的 探讨不同固定液和固定时间对大鼠睾丸组织病理学制片质量的影响。方法 选用雄性SD大鼠12只,取两侧睾丸组织,共24例标本。每例标本分别用不同的固定液(10%中性福尔马林溶液、30%Davidson固定液及FAA固定液)固定不同的时间(24、48 h)后,进行脱水、包埋、切片、HE染色。用LEICA DM5000B普通光学显微镜观察。结果 3种固定液固定24 h后,可见30% Davidsons固定液组睾丸组织的异常变化程度低于10%中性福尔马林组和FAA组;固定时间对睾丸组织有一定程度的影响:固定24 h的异常变化程度低于固定48 h。结论 30% Davidson固定液组SD大鼠睾丸组织病理学变化程度最小;固定时间不宜超过24 h。
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[Abstract]
Objective To investigate the effects of different fixatives and fixation time on testis histopathology of rats. Methods A total of 24 testis tissue samples were collected from 12 male rats. Every specimen was for dehydration, embedding, sectioning, and HE staining after fixing for 24 or 48 h in different fixatives (10% neutral buffered formalin, 30% Davidsons fixative, and FAA fixative), then the tissues were observed by LEICA DM5000B ordinary optical microscopy. Results The testis histopathology changes by 30% Davidsons fixative were less than those by 10% neutral buffered formalin and FAA fixative after fixed for 24 h; The fixation time had a certain effect on testis tissue: histopathology change of 24 h was less than that of 48 h. Conclusion Abnormal change of testis histopathology in rats in 30% Davidsons fixative was minimized; Fixation time should not be more than 24 h.
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