[关键词]
[摘要]
目的 探讨复方肠泰对葡聚糖硫酸钠(DSS)诱导的小鼠慢性溃疡性结肠炎(UC)的治疗作用。方法 随机将C57BL/6小鼠分为正常对照组、DSS模型组、复方肠泰给药组。采用2% DSS溶液对小鼠进行造模,小鼠连续5 d自由饮用DSS水,接着连续5 d自由饮水,重复3个循环。复方肠泰组每天按照18 g/kg ig给药,其他组ig给予相同体积的蒸馏水。每天记录小鼠体质量、疾病活动指数(DAI)评分结果,采用HE染色观察结直肠组织病理学变化,给予组织病理学评分;ELISA检测TNF-α、IL-10表达水平,免疫组化检测COX-2蛋白表达情况。结果 与DSS模型组比较,复方肠泰组小鼠疾病状态得到明显改善,DAI和组织病理学评分显著降低,炎症因子IL-10表达升高,TNF-α、COX-2蛋白的表达降低。结论 复方肠泰对小鼠慢性UC具有显著地治疗作用,可能通过促进抗炎症因子IL-10的表达,同时抑制促炎症因子TNF-α、COX-2蛋白的表达发挥作用。
[Key word]
[Abstract]
Objective To investigate the therapeutic effect of Fufang Changtai on dextran sulfate sodium (DSS)-induced chronic ulcerative colitis (UC) in mice. Methods C57BL/6 mice were randomly divided into three groups: Vehicle group, DSS model group, and Fufang Changtai group. The model of colitis in mice was induced by drinking water containing 2% DSS for 5 d, followed by 5 d of normal water for 3 times. In Fufang Changtai group, the mice were ig given Fufang Changtai 18 g/kg per day during DSS treatment. Mice in Vehicle group and DSS model group were given the same volume of water, instead. Body weight and disease activity index (DAI) score were recorded every day. The colorectal mucosal histological changes were observed by HE staining. Tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) levels in colorectal tissue were determined by ELISA. The expression of COX-2 was determined by immunohistochemistry. Results Compared with the DSS groups, Fufang Changtai group could significantly improve the symptoms, reduce colonic DAI and histological scores. The levels of TNF-α and COX-2 were markedly decreased accompanying with IL-10 content increasing by the treatment of Fufang Changtai. Conclusion Fufang Changtai has the therapeutic effects on DSS-induced UC in mice, which is probably mediated by the enhancement of anti-inflammatory cytokines IL-10 and the inhibition of pro-inflammatory cytokines TNF-α and COX-2.
[中图分类号]
[基金项目]
国家自然科学基金(81302968);中国中医科学院自主选题课题(ZZ08080012)