[关键词]
[摘要]
目的 建立测定不同产地西洋参药材及其产品中10 种皂苷成分含量的方法,比较不同来源西洋参中总皂苷及单体皂苷的含量差异,评价品质优劣。方法 采用高效液相色谱法,色谱条件为Thermo Scientific Syncronis C18 色谱柱(100 mm×2.1mm,1.7 μm),流动相为乙腈-水,二元梯度洗脱,体积流量为0.2 mL/min,检测波长为203 nm,柱温为35 ℃。结果 人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rd、F1、R0、F2、Rk1 分别在线性范围内存在良好的线性关系,48 h 内稳定性试验RSD 值均小于5%,平均加样回收率为96%~102%,RSD 值均< 2.21%。结论 该方法准确、快速、灵敏,重复性好,可用于西洋参药材、饮片及相关产品中皂苷的定量分析。不同来源西洋参及其产品中皂苷成分的种类相似,但单体皂苷的含量差异显著,所测样品均不含有人参皂苷Rf,排除掺假。
[Key word]
[Abstract]
Objective To establish the method to determine the contents of 10 ginsenoside compounds in Panax quinquefolium from different origins. And the contents of total ginsenosides and monomer ginsenosides in samples from different origins were compared. The quality of different sample was evaluated. Methods Thermo Scientific Syncronis C18 (2.1 mm × 100 mm, 1.7 μm) was used with a gradient of acetonitrile and 0.1% formic acid at a flow rate of 0.2 mL/min. The detection wavelength was set at 203 nm and the column temperature was at 35 ℃ . Results Ginsenoside Rg1, Re, Rb1, Rc, R0, Rb2, Rd, F1, F2, and Rk1 were in a good linear relationship within the linear range, respectively. And the average RSD value of stability test is less than 5% within 48 h. The average sample recovery was between 96%—102%. Conclusion This method is accurate, rapid, and sensitive and with good repeatability. The method can be used for quantitative analysis of ginsenosides in P. quinquefolium materials, decoction pieces, and related products. The types of ginsenoside constituents in samples from different origins were similar and the contents of monomer ginsenosides were significantly different. No ginsenoside Rf was found in the detected samples and eliminated the adulteration.
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[基金项目]
公益性行业(农业)科研专项经费项目(20130311106)