[关键词]
[摘要]
目的 研究鼠尾草酸(carnosic acid,CA)的遗传毒性。方法 选用鼠伤寒沙门杆菌回复突变(Ames 试验)、体内哺乳动物红细胞微核、精子畸形以及单细胞凝胶电泳(彗星试验)四项致突变生物学试验进行筛选评价。结果 在6.25~50μg/mL 的剂量水平,CA 对鼠伤寒沙门菌株TA97、TA98、TA100、TA102 和TA1535 均无诱变性;此外,在受试剂量下小鼠骨髓嗜多染红细胞微核、小鼠精子畸形以及体内彗星试验的结果均为阴性(与溶剂对照比较,P> 0.05)。结论 在本实验条件下,CA 未见明显遗传毒性。
[Key word]
[Abstract]
Objective To study the genotoxicity of carnosic acid (CA). Methods Salmonella Typhimurium reverse mutation assay (Ames test), in vivo mammalian erythrocyte micronucleus assay, sperm malformation assay, and single cell gel electrophoresis (Comet assay) were conducted to evaluate the genotoxicity. Results Under the concentration of 6.25—50 μg/mL, CA showed no mutagenic potency to the strains of TA97, TA98, TA100, TA102, and TA1535. Meanwhile in the test dose range, the genotoxicity indicated by mice bone marrow micronucleus assay, mice sperm malformation assay, and in vivo comet assay were negative (compared with solvent control, P>0. 0.05). Conclusion CA has no obvious genotoxicity under the test condition.
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[基金项目]
湖南中医药科研计划项目(2013106)