目的 对不同干燥方式所得地龙氨基酸组分提取物进行指纹图谱测定方法的研究, 比较不同干燥方式对地龙氨基酸组分提取物品质的影响。方法 采用异硫氰酸苯酯柱前衍生化反相高效液相色谱法, 测定并比较真空冷冻干燥、真空减压干燥和微波干燥3种方式所得地龙干燥样品以及未经干燥样品的指纹图谱, 以中药色谱指纹图谱相似度评价系统计算相似度, 并进行图谱比较和指认分析。结果 建立了不同干燥技术处理的地龙氨基酸组分提取物高效液相指纹图谱, 确定了17个共有峰, 同一干燥方法不同批次干燥产品的镜像度较高, 具有良好的相似性(>0.9), 不同干燥方法所得提取物之间有较大的质量差异。结论 HPLC指纹图谱分析法可较全面的反映不同干燥技术处理的地龙氨基酸组分提取物间的差异, 可用于同类提取物干燥工艺的质量控制和工艺评价。冷冻干燥法是地龙氨基酸组分提取物最适宜的干燥方法。
Objective To study HPLC fingerprint methods of Pheretima amino acid components extract obtained by different drying methods and compare the effect of different drying methods on the quality of Pheretima amino acid components extract. Methods After derivatization of amino acids in Pheretima extract with taking phenyl isothiocyanate as derivatization reagent, the amino acids were analyzed by HPLC fingerprint technology. Pheretima extract was dried by vacuum freeze-drying, vacuum drying, and microwave drying. The similarity of chromatograms was calculated using a professional software named Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Chinese Pharmacopoeia Committee Version 2004A). Results Seventeen common peaks were identified in the Pheretima amino acid components extract by HPLC fingerprint. The chromatograms of different batches of product dried by the same drying methods present a good similarity (> 0.9). There are certain quality differences in the extract by the different drying methods. Conclusion The quality control and process evaluation of herbal extracts by HPLC fingerprint technology are simple and reliable. The optimal drying method for Pheretima extract is vacuum freeze-drying.