目的 研究龙血竭及其总黄酮类成分对慢性血瘀证兔模型的血液流变学指标及心主动脉内皮细胞等的影响。方法 建立慢性血瘀证兔模型, 造模同时各给药组每天给药1次, 干预4周后对各组血液流变学各项指标以及血清一氧化氮(NO)和内皮素(ET)水平进行检测, 并通过HE染色病理切片对兔心主动脉内皮细胞形态学变化进行观察比较。结果 与模型组相比, 龙血竭及其总黄酮类成分均能降低慢性血瘀证兔模型全血黏度、血浆黏度、红细胞压积、红细胞聚集指数和卡森黏度, 能升高红细胞变形指数, 对血小板聚集有抑制作用;同时能降低慢性血瘀证兔模型血清ET水平, 升高NO水平;此外, 各给药组兔心主动脉HE染色病理切片内皮细胞较模型组结构完整而光滑, 排列平行有序, 损失程度有所改善。结论 龙血竭及其总黄酮类成分均能改善慢性血瘀证兔模型的血液流变学特性, 调节NO/ET平衡, 并对其心主动脉内皮细胞有保护作用。
Objective This study is designed to explore the effect of Sanguis draxonis and its total flavonoids on hemorheology index and the heart aortic endothelial cells in the rabbit model with chronic stasis syndrome. Methods The rabbit-models of chronic stasis syndrome were established. At the beginning of the model making, each dosage group was dosed once daily. Four weeks later, the indexes of hemorheology, rabbit serum nitric oxide (NO), and endothelin (ET) level were detected, and the rabbit heart aorta tissue of endothelial cells morphological changes were observed by HE staining. Results Compared with the model group, both Sanguis draxonis and its total flavonoids can reduce whole blood viscosity, plasma viscosity, erythrocyte deposited, casson viscosity, and erythrocyte aggregation index, increase erythrocyte deformation index, and inhibit the platelet aggregation in the rabbit model with chronic stasis syndrome; At the same time, it also can reduce the serum levels of ET while increase the NO level in the rabbit model with chronic stasis syndrome. In addition, each dose group of endothelial cell structures in rabbit heart aorta of HE staining were complete, smooth, parallel arranged orderly, and loss rates were improved, compared with model group. Conclusion Both Sanguis draxonis and its total flavonoids can obviously improve blood rheological properties,regulate the balance of NO/ET, and protect the aortic endothelial cells in the rabbit model with chronic stasis syndrome.