目的 探讨竹节香附素A对人肝细胞癌裸鼠移植瘤的抑制作用.方法 建立人肝癌裸鼠移植瘤模型,随机将成瘤裸鼠分为3组,即竹节香附素A治疗组、顺铂组及生理盐水对照组,于种植瘤细胞后第3天开始ip药物,治疗过程中隔天记录瘤体大小、各组抑瘤率及药物对裸鼠生存率的影响及瘤组织病理学改变.结果 竹节香附素A治疗组能明显抑制人肝癌细胞裸鼠移植瘤生长,治疗8 d竹节香附素A组及顺铂组与对照组相比已有明显差异(F=5.579,P=0.015),抑瘤率分别达到48.97%和53.71%,随着治疗时间的延长差异更加显著;治疗结束后竹节香附素A组和顺铂组平均瘤体质量较轻,与对照组相比差异具有统计学意义(F=17.96,P=0.000);与化疗药顺铂组相比瘤体大小及抑瘤率均未见差异.竹节香附素A对裸鼠体质量影响较小,治疗结束后竹节香附素A治疗组裸鼠存活率为100%,顺铂治疗组存活率仅为62.5%,两组存活率相比差异有显著性(P<0.05).病理学观察竹节香附素A治疗组瘤体小,肿瘤细胞稀疏减少.结论 竹节香附素A对人肝癌裸鼠移植瘤生长具有明显抑制作用,且竹节香附素A毒性低,安全性好.

Objective To study the inhibiting effect of raddeanin A on xenograft tumor in nude mice with transplanted human hepatocellular carcinoma. Methods The xenograft tumor modes of human HCC were established in nude mice. These nude mice were randomly divided into three groups (raddeanin A, cisplatin, and normal saline). In all groups, intraperitoneal injection (1 time per day) of drugs will be given from the third day after transplanted human HCC. During the treatment, all groups' date of tumor size and tumor inhibition rate were recorded, every other day. To anlayze drugs' impact on the survival rate and the histopathological changes of xenograft tumor. Results (1) This study finds that raddeanin A has significant inhibitive effect on xenograft tumor in nude mice. There was statistical significant difference among raddeanin A treatment group, cisplatin treatment group and normal saline control group on the 8th day (F = 5.579, P = 0.015). In the former two treatment groups, the tumor inhibition rate were 48.97% and 53.71% at that day. With the treatment prolonged, the differences was more significant. The average weight of tumor is lighter in raddeanin A group and Cisplatin group, Which have statistically significant compared with the normal saline control group after treatment (F=17.96,P=0.000). Whereas the difference in tumor size and tumor inhibition rate between raddeanin A treatment group and cisplatin treatment group had not statistical significance(P > 0.05). (2) Raddeanin A had small bad effect on the nude mice physical: after treatment, survival rate of nude mice was 100% in raddeanin A treatment group and 62.5% in cisplatin treatment group, a significant difference between the two groups (P < 0.05). (3) Pathological observation of raddeanin A treatment group showed that raddeanin A could shrink the tumors and make tumor cells were arranged loosely. Conclusion These finds reveal that raddeanin A, with the advantage of mild toxicity and high safety, has significant inhibitive effect on xenograft tumor in nude mice with transplanted human hepatocellular carcinoma.

福建省自然科学基金项目(NO2012J01399)