[关键词]
[摘要]
目的 建立HPLC法测定龙加通络胶囊中薯蓣皂苷。方法 采用Diamonsil C18(4.6 mm×250 mm,5 mm)色谱柱,流动相为乙腈-水(55:45),体积流量1.0 mL/min,设置温度90 ℃,气流量2.4 L/min,柱温35 ℃。结果 薯蓣皂苷在1.03~6.18 μg与峰面积呈良好的线性关系(r=0.999 8),平均回收率100.96%,RSD=1.97%。结论 本方法简便、准确、重复性好,可用于龙加通络胶囊中薯蓣皂苷的质量控制。
[Key word]
[Abstract]
Objective To establish a method for the content determination of dioscin in Longjia Tongluo Capsule by HPLC. Methods The Diamonsil-C18 (250 mm ×4.6 mm, 5 mm) column was used. The mobile phases consisted of acetonitrile and water (55:45); The flow rate was 1.0 mL/min, the set temperature was 90 ℃, the gas flow was 2.4 L/min, and the column temperature was 35 ℃. Results The linear range of dioscin was 1.03—6.18 μg (r=0.999 8), the average recovery was 100.96%, with RSD of 1.97%. Conclusion The method is simple, accurate, and reproducible, and it can be used in the quality control in Longjia Tongluo Capsule.
[中图分类号]
[基金项目]