目的 优选和胃颗粒中槟榔碱的最佳提取工艺。方法 应用单因素试验考察药材饮片的浸泡时间及吸水率,以槟榔碱提取率和出膏率的综合评分为指标,通过正交试验考察加水量、煎煮时间、煎煮次数对和胃颗粒提取工艺的影响,并采用高效液相色谱法测定槟榔碱的含量及其溶出度。结果 和胃颗粒中槟榔碱的最佳提取工艺为加水煎煮2次,第一次加水6倍量,煎煮1 h,趁热滤过;第二次加水5倍量,煎煮1 h,趁热滤过,合并滤液。结论 该提取工艺合理、可靠,适用于和胃颗粒的工业生产。

Objective To optimize the extraction technology for arecoline from Hewei Granules. Methods The method of single factor test was used to inspect soaking time and water absorption, with the yield of extract and the content of arecoline as indexes. Orthogonal test was adopted to investigate the influence of the amount of water, decocting time and frequences. HPLC was adopted to determine the content and dissolution of arecoline. Results The best extraction technology for arecoline from Hewei Granules was as follows: to be decocted twice, six times the amount of water for the first time, decoction for 1 h, hot filtration; five times the amount of water for the second time, decoction for 1 h, hot filtration and the filtrate was combined. Conclusion The extraction technology is reasonable, reliable, and suitable for the industrial production of Hewei Granules.