目的 观察杜仲提取物对缺血再灌注损伤大鼠脑组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)及诱导型一氧化氮合酶(iNOS)mRNA表达的影响。方法 采用大鼠大脑中动脉线栓法制备局灶性脑缺血再灌注损伤模型,造模前以杜仲提取物不同剂量连续ig给药14 d,缺血2 h,再灌注24 h后,观察杜仲提取物对脑缺血再灌注损伤大鼠神经功能症状,脑梗死范围改变,实时荧光定量PCR(RT-PCR)法检测脑缺血再灌注损伤大鼠海马TNF-α、IL-1β及iNOS mRNA的表达。结果 杜仲提取物可显著改善脑梗死范围(P<0.05),下调海马TNF-α、IL-1β及iNOS mRNA的表达(P<0.05)。结论 杜仲提取物可下调大鼠脑组织中TNF-α、IL-1β及iNOS mRNA的表达产生抗局灶性脑缺血再灌注损伤作用。

Objective To investigate the effects of Eucommiae Cortex extracts on tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and inducible nitric oxide synthase (iNOS) mRNA expression in cerebral ischemia-reperfusion injury of rats. Methods The cerebral ischemia-reperfusion injury was induced by thread block of the middle cerebral artery. Eucommiae Cortex extracts were ig administrated once daily for consecutive 14 d pretreatment. After 24 h of ischemia and reperfusion, the neurologica1 deficit score was assessed and the brain tissues were obtained for detecting. The morphology of the brain tissues was observed with 2, 3, 5-triphenyl tetrazolium chloride (TTC) staining. Real time PCR was used to determine the mRNA expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and inducible nitric oxide synthase (iNOS). Results Compared with the model group, Eucommiae Cortex extracts ccould significantly decrease the brain infarction range and down-regulate the expression of TNF-α, IL-1β and iNOS mRNA. Conclusion Eucommiae Cortex extracts have the protective effects on focal brain ischemia reperfusion injury in rats by down-regulatting the expression of TNF-α, IL-1β, and iNOS mRNA.

贵州省中医药管理局资助项目(黔中医药发 [2011] 78号)