目的 研究苗药黑骨藤镇痛抗炎作用的活性部位。方法 小鼠随机分为空白组、阿司匹林组及黑骨藤水、正丁醇、氯仿、醋酸乙酯、石油醚提取部位的低、高剂量组。除空白组外，各组ig给药，空白组ig生理盐水。采用扭体法观察记录给药后15 min内小鼠扭体次数。采用热板法测定小鼠的痛觉反应时间。采用耳廓肿胀法检测小鼠耳廓肿胀度。采用棉球肉芽肿法：观测小鼠肉芽湿质量和干质量。结果 黑骨藤各提取部位对醋酸诱发小鼠扭体反应有显著的镇痛作用，其中黑骨藤正丁醇提取部位高剂量组效果最好，其疼痛抑制率为80.2%。黑骨藤正丁醇提取部位对热板法致痛有显著的镇痛作用，与空白组比较，能显著提高小鼠的痛阈值（P<0.05、0.01）。黑骨藤水、醋酸乙酯、石油醚提取部位对二甲苯致小鼠耳廓肿胀的急性炎症有显著的抗炎作用，能明显减少小鼠耳廓肿胀度。黑骨藤各提取部位对小鼠肉芽湿质量和干质量均有抑制作用，其中正丁醇提取部位效果最好。结论 黑骨藤具有良好的镇痛抗炎作用。其中，正丁醇部位镇痛作用最好。

Objective To study the analgesic and anti-inflammatory constituents from Miao medicine Periploca forrestii. Methods Mice were randomly divided into the normal, aspirin, high- and low-dose of petroleum ether extract, ethyl acetate extract, trichloromethane extract, butyl alcohol extract, and water extract of P. forrestii groups. ① Twisting test:the time of twisting of mice was observed within 15 min. ② Hot-plate test:female mice were ig given relevant medicinals and the time of pain reaction was determined by using hot-plate test after the second and the third time ig administration of 30 min. ③ Ear swelling test: mouse auricle tumefaction was induced by dimethylbenzene and the mass of auricle was tested. ④ Cotton ball-granulation test: the mass of granulation was tested. Results ① All the extract fractions of P. forrestii had the significant analgesic effects on acetic acid-induced twisting reaction in mice. Compared with the normal group, the times of twisting decreased significantly in butyl alcohol extrac groups. ② In butyl alcohol groups, the significant analgesic effects on hot-plate-related pain were observed and pain thres hold was increased significantly compared with the normal group (P < 0.05 or P < 0.01). ③ Butyl alcohol extrac could not have the inhibition effects on auricle swelling caused by xylene in mice. ④ All the extract fractions of P. forrestii had the significant inhibition effects on granulation caused by cotton ball in mice. Compared with the normal group, the bass of granulation decreased significantly in butyl alcohol extrac groups. Conclusion P. forrestii has the anti-inflammation and the analgesic effects, and butyl alcohol extract fractions of P. forrestii has the significantly analgesic and anti-cheonic inflammation effects.

贵州省中药现代化科技产业研究开发专项项目（黔科合ZY字 [2013] 3003号）；贵州省科技技术基金项目（黔科合J字 [2012] 2069号）；贵阳市科技局科技创新公共技术平台建设项目（筑科合同 [2010] 3-2号）；贵阳中医学院博士基金项目；贵阳中医学院“大学生创新性实验”项目