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[摘要]
目的 建立安尔眠胶囊中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(C20H2209)的含量测定方法。方法 采用高效液相色谱(HPLC)法,以Dionex Acclaim 120® C18色谱柱(150 mm×4.6 mm,5 μm)为分离柱,以乙腈-1%甲酸溶液(23:77)为流动相,体积流量1.0 mL/min,检测波长320 nm,柱温25 ℃。结果 2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在0.010~0.200 μg呈现良好的线性关系(r=0.999 6),平均回收率为97.35%,RSD为2.07%。结论 该方法<准确可靠,适用于安尔眠胶囊中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量测定。
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[Abstract]
Objective To establish the method for the content determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Anermian Capsule by HPLC. Methods The chromatographic condition was Dionex Acclaim 120® C18 column (150 mm × 4.6 mm, 5 μm), mobile phase was acetonitrile-water (containing 1% formic acid) (23:77), flow rate was 1.0 mL/min, detective wavelength was 320 nm, and column temperature was 25 ℃. Results 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside had a good linear relationship in the range of 0.010—0.200 μg (r = 0.999 6), and the mean recovery rate was 97.35% (RSD = 2.07%). Conclusion The method is accurate and reliable for determination of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in Anermian Capsule.
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