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[摘要]
目的 探索建立超快速液相色谱(UFLC)法测定复方丹参片中三七皂苷R1、人参皂苷Rg1及Rb1。方法 采用SHIMADZU Shim-pack XR-ODS Ⅲ(2.0 mm×75 mm, 1.6 μm)色谱柱;以乙腈-水作为流动相进行梯度洗脱;体积流量为0.4 mL/min;检测波长为203 nm;进样体积为3 μL。结果 三七皂苷R1、人参皂苷Rg1及Rb1分别在0.025 7~0.257 0、0.101 2~1.012 0、0.104 4~1.044 0 μg与峰面积呈良好的线性关系,平均加样回收率分别为96.7%、98.1%、98.8%。结论 本方法在15min内可以将三七皂苷R1、人参皂苷Rg1及Rb1有效分离,节省了大量人力和流动相的消耗,为中药的质量控制技术提供参考方法。
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[Abstract]
Objective To establish the quantitative method of notoginsenoside R1, ginsenosides Rg1 and Rb1 in Compound Danshen Tablet by ultra-fast liquid chromatography (UFLC). Methods This assay was performed on Shimadzu Shim-pack XR-ODS Ⅲ (75 mm × 2.0 mm, 1.6 μm) column with acetonitrile-water as mobile phase in gradient elution at a flow rate of 0.4 mL/min; And the detection wavelength was 203 nm. The injection volume was 3 μL. Results The linear of notoginsenoside R1, ginsenosides Rg1 and Rb1 had good linearity within the range of 0.025 7—0.257, 0.101 2—1.012, and 0.104 4—1.044 μg. And their average recovery ratios were 96.7%, 98.1%, and 98.8%. Conclusion UFLC method may greatly improve the separation efficiency and analysis speed in the case of notoginsenoside R1, ginsenosides Rg1 and Rb1 in Compound Danshen Tablet while reducing the solvent consumption. As an alternative of conventional HPLC, UPLC is more convenient, fast, and feasible.
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